A SIMPLE METHOD FOR EMBEDDING SMALL SPECIMENS FOR PHOTOMICROGRAPHY AND SECTIONING FOLLOWING INTRACELLULAR MICROIONTOPHORESIS OF LUCIFER YELLOW CH

被引：2

作者：

JONES, CJ ^{[1
]}

机构：

[1] UNIV DURHAM, SCI LABS, DEPT ZOOL, DURHAM DH1 3LE, ENGLAND

来源：

HISTOCHEMICAL JOURNAL | 1986年 / 18卷 / 2-3期

关键词：

D O I：

10.1007/BF01675363

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A simple method for the rapid processing of small specimens following intracellular labelling with the fluorescent naphthalimide dye Lucifer Yellow CH is described which involves embedding in glycol methacrylate-based resin on cavity slides. The technique, which may be suitable for other intracellular and extracellular fluorescent markers, permits early fluorescence photomicrography of wholemounts and subsequent recovery of the specimens of serial sectioning and further analysis. In the present study on isolated human eccrine sweat glands, the procedure has facilitated both the identification of cells from which electrical records have been made and the determination of their dye-coupling status.

引用

页码：105 / 108

页数：4

共 12 条

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