SIMULTANEOUS DETERMINATION OF THEOPHYLLINE AND CAFFEINE BY REVERSED PHASE LIQUID-CHROMATOGRAPHY USING PHENYL COLUMN

被引：7

作者：

WONG, SHY ^{[1
]}

MARZOUK, N ^{[1
]}

MCHUGH, SL ^{[1
]}

CAZES, E ^{[1
]}

机构：

[1] IBM INSTRUMENTS INC, DANBURY, CT 06810 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1985年 / 8卷 / 10期

关键词：

BIOMEDICAL ENGINEERING - Patient Treatment;

D O I：

10.1080/01483918508074096

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phenyl, C-8, and C-18 columns were evaluated for the simultaneous analysis of theophylline (TH) and caffeine (CA). The phenyl column was chosen for its capability of resolving of 1,7-dimethylxanthine from theophylline. The procedure utilized protein precipitation of either 50 or 100 mu l of serum with diluted trichloroacetic acid, followed by analysis with either a manual isocratic or automated gradient elution mode. Chromatographic conditions were: column equals phenyl (4. 5 multiplied by 150 mm with a 4. 5 multiplied by 50 mm guard column), mobile phase equals phosphate or acetate/ACN, and detection wavelength equals 280 nm.

引用

页码：1797 / 1816

页数：20

共 25 条

[1] METHYLXANTHINES IN APNEA OF PREMATURITY [J].