GLUTAMINE LIMITED FED-BATCH CULTURE REDUCES AMMONIUM ION PRODUCTION IN ANIMAL-CELLS

被引：25

作者：

LJUNGGREN, J ^{[1
]}

HAGGSTROM, L ^{[1
]}

机构：

[1] ROYAL INST TECHNOL,DEPT BIOCHEM & BIOTECHNOL,S-10044 STOCKHOLM 70,SWEDEN

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 10期

关键词：

D O I：

10.1007/BF01024725

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In a glutamine limited fed-batch culture of the murine myeloma cell Sp 2/0-Ag 14 the production of ammonium ions was reduced to half of that produced in an ordinary batch culture. Other parameters like μ, DOT, length of stationary phase, the quotients lactate/glucose, ammonium/glutamine, and alanine/glutamine were also influenced by the feeding technique. © 1990 Kluwer Academic Publishers.

引用

页码：705 / 710

页数：6

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