Simple screening method for lipase for transesterification in organic solvent

被引：30

作者：

Furutani, T ^{[1
]}

Su, RH ^{[1
]}

Ooshima, H ^{[1
]}

Kato, J ^{[1
]}

机构：

[1] OSAKA CITY UNIV,FAC ENGN,DEPT BIOAPPL CHEM,SUMIYOSHI KU,OSAKA 558,JAPAN

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 12期

关键词：

lipase; substrate specificity; p-nitrophenyl esters; hydrolysis; transesterification; organic solvent;

D O I：

10.1016/0141-0229(95)00035-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated lipase-catalyzed hydrolysis in water and dioxane-water with a simple colorimetric method. We screened 24 lipases for the ability to hydrolyze p-nitrophenyl esters as chromogenic substrates. Their hydrolytic activities were varied by adding dioxane. Most of the lipases showed high activity in hydrolysis in water, but some showed activity in 50% dioxane-water several tens times higher than those in water. Moreover, several lipases with hydrolytic abilities in 50% dioxane-water also catalyzed the transesterification of p-nitrophenol using fatty acid vinyl esters. We found it possible that a useful lipase for transesterification can be selected by measuring the hydrolysis activity of p-nitrophenyl ester in 50% dioxane-water.

引用

页码：1067 / 1072

页数：6

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