DIFFERENTIAL REGULATION OF CD43 GLYCOFORMS ON CD4(+) AND CD8(+) T-LYMPHOCYTES IN GRAFT-VERSUS-HOST DISEASE

被引:33
作者
ELLIES, LG [1 ]
JONES, AT [1 ]
WILLIAMS, MJ [1 ]
ZILTENER, HJ [1 ]
机构
[1] UNIV BRITISH COLUMBIA,DEPT PATHOL & LAB MED,VANCOUVER,BC V6T 1Z3,CANADA
基金
英国医学研究理事会;
关键词
ACTIVATION; CD43; GLYCOSYLTRANSFERASES; GRAFT-VERSUS-HOST DISEASE; T LYMPHOCYTES;
D O I
10.1093/glycob/4.6.885
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two distinct T-cell glycoforms of CD43 result from differential glycosylation of a single gene product in vivo. The 115 kDa glycoform tarries mainly tetrasaccharides and is a pan T-cell marker, whereas the 130 kDa glycoform carries mainly hexasaccharides and is associated with T-cell activation, CD43 has been shown to play a role both in enhancing and inhibiting cell adhesion; however, the function of the individual glycoforms is unknown. We have examined the distribution and regulation of the CD43 glycoforms in a murine model of acute graft-versus-host disease (GVHD) using monoclonal antibodies (mAbs) S7 and 1B11 specific for the 115 and 130 kDa CD43 glycoforms, respectively, An increase in T-lymphocyte CD43 130 kDa expression occurred during GVHD from day 4 onwards and coincided with splenomegaly and upregulation of the beta 1-6GlcNAc transferase (C2GnT), the key enzyme responsible for the addition of complex O-glycan branching to CD43. When T-lymphocyte subsets were examined for CD43 expression, we found that in GVHD, both CD43 glycoforms were upregulated on CD4(+) T cells. However, in CD8(+) T cells, CD43 115 kDa was downregulated while CD43 130 kDa was dramatically upregulated, such that two distinct CD8(+)1B11(+) T-cell subsets were observed. These data demonstrate differential expression of the CD43 glycoforms in both resting and activated CD4(+) and CD8(+)T cells, and suggest that glycosylation differences between the CD43 glycoforms may reflect participation in the different functions of these T-cell subsets in immune disorders in vivo.
引用
收藏
页码:885 / 893
页数:9
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