PHENOLIC ACCUMULATION AND PEROXIDASE-ACTIVITY IN INVITRO SELECTED ALFALFA CALLUS-CULTURES RESISTANT TO FILTRATE OF FUSARIUM SPP

被引：7

作者：

HRUBCOVA, M

CVIKROVA, M

MERAVY, L

EDER, J

BINAROVA, P

机构：

[1] Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6, 160 00

[2] Institute of Experimental Botany, Czechoslovak Academy of Sciences, Olomouc, 772 00

来源：

BIOLOGIA PLANTARUM | 1992年 / 34卷 / 3-4期

关键词：

D O I：

10.1007/BF02925869

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Changes in the phenylalanine ammonia-lyase (PAL) activity, accumulation of phenolic acids and ionically-bound peroxidase activity in the in vitro selected embryogenic and nonembryogenic Medicago sativa callus cultures resistant to the filtrate of Fusarium spp. were found. The PAL activity in both in vitro selected cultures during a 4-week cultivation on a medium with phytotoxins was higher than in the control calli grown on a medium without toxin. The filtrate from Fusarium spp. evoked an increase in the contents of all determined phenolic acids in the selected calli. They occurred predominantly bound as esters. The most pronounced portions in the elevated acids level were of ester-bound p-hydroxybenzoic, vanillic, p-coumaric and ferulic acids. The ionic cell wall-bound peroxidase activity in both selected calli cultivated on a medium with a filtrate was twice as high as the activity determined in the control cultures. The activity of soluble peroxidase was not influenced by challenge with a filtrate. No significant differences were found between the in vitro selected embryogenic and nonembryogenic alfalfa callus cultures in the response to the phytotoxic filtrate.

引用

页码：203 / 211

页数：9

共 31 条

[1]

Bajaj K.L., Singh P.P., Kaur G., Effect of cercosporin toxin on polyphenol metabolism in mungbean (Vigna radiata L. Wilczek) leaves, Biochemie und Physiologie der Pflanzen, 180, pp. 621-624, (1985)

[2]

Beaudoin-Eagan L.D., Thorpe T.A., Turnover of shikimate pathway metabolites during shoot initiation in tobacco callus cultures, Plant Cell Physiol., 25, pp. 913-921, (1984)

[3]

Binarova P., Novak J.F., Studies on embryogenesis into different types of cell cultures of Medicago sativa, Acta Univ. Agr., 23, pp. 293-296, (1985)

[4]

Binarova P., Nedelnik J., Fellner M., Nedbalkova B., Selection for resistance to filtrates of Fusarium spp. in embryogenic cell suspension culture of Medicago sativa L, Plant Cell Tissue Organ Cult., 22, pp. 191-196, (1990)

[5]

Blaydes D.F., Interaction of kinetin and various inhibitors of the growth of soybean tissue, Physiologia Plantarum, 19, pp. 748-753, (1966)

[6]

Bolwell G.P., Robbins M.O., Dixon R.A., Metabolic changes in elicitor-treated bean cells. Enzymic responses associated with rapid changes in cell wall components, Eur. J. Biochem., 148, pp. 571-578, (1985)

[7]

Bray H.C., Thorpe W.V., Determination of phenols, Methods of Biochemical Analysis, pp. 27-52, (1954)

[8]

Castillo F.J., Penel C., Greppin H., Peroxidase release induced by ozone in Sedum album leaves, Plant Physiol., 74, pp. 846-851, (1984)

[9]

Cvikrova M., Hrubcova M., Meravy L., Pospisil F., Changes in the content of phenolic substances during the growth of Nicotiana tabacum cell suspension culture, Biol. Plant., 30, pp. 185-192, (1988)

[10]

Dixon R.A., The phytoalexin response: Elicitation, signalling and the control of host gene expression, Biological Reviews, 61, pp. 239-291, (1986)

← 1 2 3 4 →