APPARENT DESENSITIZATION OF NMDA RESPONSES IN XENOPUS OOCYTES INVOLVES CALCIUM-DEPENDENT CHLORIDE CURRENT

被引：122

作者：

LEONARD, JP

KELSO, SR

机构：

[1] Department of Biological Sciences University of Illinois at Chicago, Chicago

来源：

NEURON | 1990年 / 4卷 / 01期

关键词：

D O I：

10.1016/0896-6273(90)90443-J

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

N-Methyl-d-aspartate (NMDA) receptors were expressed and studied in Xenopus oocytes injected with rat brain RNA. NMDA application elicits a rapid inward current that decays in several seconds to a relatively stable level. This decay is reportedly due to desensitization. However, we found the early transient component could be evoked more than once during a single application of NMDA, suggesting that the receptor did not actually desensitize. Removal of external Ca2+, replacement of Ca2+ with Ba2+, or intracellular injection of EGTA abolished the transient component. Furthermore, a variety of CI- channel blockers nearly eliminated the transient component and inhibited the plateau current as well. We propose that a significant portion of the NMDA current recorded in oocytes is carried by a transient inward Cl- current triggered by Ca2+ influx through the NMDA receptor/channel. © 1990.

引用

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页码：53 / 60

页数：8

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