Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats

被引：9

作者：

Choi, Seok ^{[1
]}

Shin, Hye Rang ^{[2
]}

Kim, Sang Hoon ^{[2
]}

Lee, Mi Jung ^{[1
]}

Jun, Jae Yeoul ^{[1
]}

Kim, Hyun Lee ^{[3
]}

Chung, Jong Hoon ^{[3
]}

Yeum, Cheol Ho ^{[1
]}

机构：

[1] Chosun Univ, Coll Med, Dept Physiol, 309 Pilmundaero, Gwangju 501759, South Korea

[2] Chosun Univ, Coll Med, Dept Psychiat, Gwangju, South Korea

[3] Chosun Univ, Coll Med, Dept Internal Med, Gwangju, South Korea

来源：

KIDNEY RESEARCH AND CLINICAL PRACTICE | 2014年 / 33卷 / 01期

关键词：

Endothelium; Renal hypertension; Superoxide; Oxidative stress;

D O I：

10.1016/j.krcp.2013.12.001

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Endothelial dysfunction is linked to exaggerated production of superoxide anions. This study was conducted to examine the effects of oxidative stress on endothelial modulation of contractions in chronic two-kidney, one-clip (2K1C) renal hypertensive rats. Methods: The 2K1C hypertension was induced by clipping the left renal artery; age-matched rats receiving sham treatment served as controls. Thoracic aortae were isolated and mounted in tissue baths for measurement of isometric tension. Results: Norepinephrine-induced contraction was augmented by the removal of the endothelium, which was more pronounced in sham rats than in 2K1C rats. Nco-nitroL-arginine methyl ester, an inhibitor of nitric oxide production, had a similar augmenting effect. Vitamin C inhibited the contraction in aortic rings with intact endothelium from 2K1C rats but not from sham rats. The contraction was also suppressed by treatment with diphenyleneiodonium or apocynin, inhibitors of nicotinamide adenine dinucleotideinicotinamide adenine dinucleotide phosphate (NADH/NADPH) oxidase, in the aortae with intact endothelium from 2K1C rats but not in those from sham rats. Superoxide anions generated by xanthine oxidaseihypoxanthine enhanced the contraction in the aortae with intact endothelium from sham rats, but had no effect in 2K1C rats. Enhanced contractile responses to norepinephrine by xanthine oxidaseihypoxanthine in sham rats were reversed by vitamin C. Conclusion: These results suggest that the effect on endothelial modulation of endothelium-derived nitric oxide is impaired in 2K1C hypertension. The impairment is, at least in part, related to increased production of superoxide anions by NADH/ NADPH oxidase. (C) 2014. The Korean Society of Nephrology. Published by Elsevier.

引用

页码：19 / 25

页数：7

共 29 条

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