QUANTITATIVE-ANALYSIS OF GIBBERELLINS BY ISOTOPE-DILUTION MASS-SPECTROMETRY - A COMPARISON OF THE USE OF CALIBRATION CURVES, AN ISOTOPE-DILUTION FIT PROGRAM AND ARITHMETICAL CORRECTION OF ISOTOPE RATIOS

被引：21

作者：

CROKER, SJ ^{[1
]}

GASKIN, P ^{[1
]}

HEDDEN, P ^{[1
]}

MACMILLAN, J ^{[1
]}

MACNEIL, KAG ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH CHEM,BRISTOL BS8 1TS,AVON,ENGLAND

来源：

PHYTOCHEMICAL ANALYSIS | 1994年 / 5卷 / 02期

关键词：

QUANTITATIVE ANALYSIS; ISOTOPE DILUTION; GAS CHROMATOGRAPHY MASS SPECTROMETRY; GIBBERELLINS; PISUM-SATIVUM L;

D O I：

10.1002/pca.2800050206

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantitative analysis of gibberellin A1 (GA1) in apices of 19-day-old seedlings of Pisum sativum L. cultivar Alaska is described using isotope dilution mass spectrometry. Three methods are compared for determining the dilution of isotope in the internal standards, [17-H-2(2)]GA1 and [2beta-H-2(1)]GA1. Similar results were obtained with each method. The use of calibration curves gave the most precise determination with [H-2(2)]GA1 as internal standard irrespective of the amount added. The use of an isotope dilution fit program was more precise using [H-2(1)]GA1 as internal standard. A simpler arithmetical correction procedure gave consistently higher estimates with both internal standards. The relative merits of the methods are discussed and the requirements for reliable quantification by isotope dilution gas chromatography-mass spectrometry are presented.

引用

页码：74 / 80

页数：7

共 18 条

[1] DETECTION OF ENDOGENOUS GIBBERELLINS AND THEIR RELATIONSHIP TO HYPOCOTYL ELONGATION IN SOYBEAN SEEDLINGS [J].