NEUTRALIZATION ENZYME-IMMUNOASSAY FOR INFLUENZA-VIRUS

被引：28

作者：

BENNE, CA ^{[1
]}

HARMSEN, M ^{[1
]}

DEJONG, JC ^{[1
]}

KRAAIJEVELD, CA ^{[1
]}

机构：

[1] NATL INST PUBL HLTH & ENVIRONM PROTECT,DEPT VIROL,BILTHOVEN,NETHERLANDS

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 04期

关键词：

D O I：

10.1128/JCM.32.4.987-990.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A neutralization enzyme immunoassay (N-EIA) was developed for the detection of antibody titer rises in sera of patients infected with influenza A (H3N2) virus. In this N-EIA, a selected strain of influenza A (H3N2) virus was added to monolayers of LLC-MK2 cells in microtiter plates. After 24 h, the replicated virus could be demonstrated with a virus-specific enzyme-labeled monoclonal antibody. Preincubation of the influenza virus with convalescent-phase sera of patients infected with influenza A (H3N2) virus resulted 1 day later in decreased absorbance values that could be used for calculation of neutralization titers. From use of paired serum samples from 10 patients with a history of flu-like symptoms, the results obtained with N-EIA correlated well (r = 0.83) with those of the standard hemagglutination inhibition test.

引用

页码：987 / 990

页数：4

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