THE AMPHICRINE PANCREATIC-CELL LINE, AR42J, SECRETES GABA AND AMYLASE BY SEPARATE REGULATED PATHWAYS

被引：37

作者：

AHNERTHILGER, G ^{[1
]}

WIEDENMANN, B ^{[1
]}

机构：

[1] FREE UNIV BERLIN,GASTROENTEROL ABT,MED KLIN & POLIKLIN,W-1000 BERLIN 33,GERMANY

来源：

FEBS LETTERS | 1992年 / 314卷 / 01期

关键词：

GABA; SMALL SYNAPTIC VESICLE; AMYLASE; REGULATED SECRETION; AR42J CELL;

D O I：

10.1016/0014-5793(92)81457-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of AR42J cells with dexamethasone leads to an enhanced formation of amylase-containing granules and facilitates their regulated secretion. Besides the exocrine properties. AR42J cells possess a specific uptake system for [H-3]GABA. The stored GABA can be released upon potassium depolarisation in a Ca2+-dependent manner. After treatment with dexamethasone, potassium depolarisation fails to release GABA, but instead causes a Ca2+-dependent secretion of amylase. Since vesicles similar to small synaptic vesicles of neurons have been identified in AR42J cells, we suggest that the regulated GABA release is mediated by this vesicle type. It is tentatively speculated that other epithelial cells, which also contain small synaptic vesicles and amino acid neurotransmitters, may release them in a similar fashion.

引用

页码：41 / 44

页数：4

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