STRUCTURE OF THE EXTRACELLULAR POLYSACCHARIDE SECRETED BY RHIZOBIUM-LEGUMINOSARUM VAR PHASEOLI CIAT-899

被引:36
作者
GILSERRANO, A [1 ]
DELJUNCO, AS [1 ]
TEJEROMATEO, P [1 ]
MEGIAS, M [1 ]
CAVIEDES, MA [1 ]
机构
[1] UNIV SEVILLA,FAC FARM,DEPT MICROBIOL & PARASITOL,E-41071 SEVILLE,SPAIN
关键词
D O I
10.1016/0008-6215(90)84025-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of the extracellular polysaccharide secreted by Rhizobium leguminosarum var. phaseoli CIAT 899 has been studied by methylation analysis, 1H-n.m.r. spectroscopy, and partial acid hydrolysis. The repeating unit is an octasaccharide made up of d-glucose, d-galactose, pyruvic acid, and acetic acid in the molar ratios 6:2:1.5:1.5. Half of the terminal Gal groups are 4,6-substituted by pyruvic acid acetal groups and the other half by O-acetyl groups at position 3. Also, one of the 3-linked glucosyl residues carries a pyruvic acid 4,6-acetal group and one of the 4-linked glucosyl residues is acetylated at position 6. © 1990.
引用
收藏
页码:103 / 107
页数:5
相关论文
共 10 条
[1]  
AMAM P, 1982, CARBOHYD RES, V103, P77
[2]   STRUCTURAL STUDIES ON EXTRACELLULAR ACIDIC POLYSACCHARIDES SECRETED BY 3 NON-NODULATING RHIZOBIA [J].
AMEMURA, A ;
HARADA, T .
CARBOHYDRATE RESEARCH, 1983, 112 (01) :85-93
[3]   A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS [J].
BLAKENEY, AB ;
HARRIS, PJ ;
HENRY, RJ ;
STONE, BA .
CARBOHYDRATE RESEARCH, 1983, 113 (02) :291-299
[4]  
Downs F., 1976, GEN METHODS GLYCOSAM, P241
[5]  
DUDMAN WF, 1983, CARBOHYD RES, V117, P141, DOI 10.1016/0008-6215(83)88083-5
[6]  
DUDMAN WF, 1983, CARBOHYD RES, V117, P169, DOI 10.1016/0008-6215(83)88085-9
[7]   USE OF POTASSIUM TERT-BUTOXIDE IN THE METHYLATION OF CARBOHYDRATES [J].
FINNE, J ;
KRUSIUS, T ;
RAUVALA, H .
CARBOHYDRATE RESEARCH, 1980, 80 (02) :336-339
[8]   HOST-SYMBIONT INTERACTIONS .12. THE STRUCTURE OF THE ACIDIC POLYSACCHARIDE SECRETED BY RHIZOBIUM-PHASEOLI STRAIN-127K44 [J].
FRANZEN, LE ;
DUDMAN, WF ;
MCNEIL, M ;
DARVILL, AG ;
ALBERSHEIM, P .
CARBOHYDRATE RESEARCH, 1983, 117 (JUN) :157-167