EFFECTS OF PROCESSING AND STORAGE ON THE MEASUREMENT OF NITROGENOUS COMPOUNDS IN OVINE BLOOD

被引：53

作者：

LABORDE, CJ ^{[1
]}

CHAPA, AM ^{[1
]}

BURLEIGH, DW ^{[1
]}

SALGADO, DJ ^{[1
]}

FERNANDEZ, JM ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,CTR AGR,DEPT ANIM SCI,BATON ROUGE,LA 70803

来源：

SMALL RUMINANT RESEARCH | 1995年 / 17卷 / 02期

关键词：

NITROGENOUS METABOLITES; BLOOD STORAGE; SHEEP; FREEZING; ANTICOAGULANTS;

D O I：

10.1016/0921-4488(95)00665-8

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Four experiments were conducted to evaluate effects of processing and storage of sheep blood samples on measurements of ammonia N (AMN), urea N (URN), total protein (TP), albumin (ALB), total globulin (GLB), and the non-albumin non-globulin protein fraction (NAG). Experiment 1 compared the effects of using different anticoagulants. Blood samples (n = 15) were collected via jugular venipuncture into one of four evacuated tubes: no anticoagulant, serum; sodium heparin, plasma; sodium fluoride and potassium oxalate, plasma; tripotassium-ethylenediaminetetraacetic acid (EDTA), plasma. Anticoagulant affected (P < 0.0001) metabolite values, with TP, ALB, and GLB being higher in heparinized plasma, and URN, NAG, and AMN highest in serum. In addition, blood packed-cell volume was 12% and 15% higher (P < 0.0001) in samples collected in heparin and EDTA compared with samples collected in sodium fluoride and potassium oxalate. Experiment 2 investigated the effects of repetitive freezing and thawing (0-3 thaw cycles) of plasma samples (n = 15 blood samples collected in tubes containing sodium fluoride and potassium oxalate). Repetitive freezing and thawing decreased (P < 0.0001) URN and NAG by 21% and 54%, respectively, the latter as a result of a 4-13% increase (P < 0.0006) in ALB and GLB values. Experiment 3 evaluated the effects of storage length (0-26 weeks) and freezing temperatures (-20 degrees C vs. -80 degrees C) on plasma samples (n = 16-20 blood samples collected in tubes containing sodium fluoride and potassium oxalate). All nitrogenous metabolites were affected by length of storage (P < 0.0001). Plasma AMN, URN, TP, and NAG were affected (0.0001 < P < 0.10) by storage temperature, and AMN, TP, GLB, and NAG measurements exhibited a temperature X day interaction (P < 0.05). In general, TP and NAG values declined with storage length, whereas URN, GLB, and AMN values increased. Plasma AMN increased 74% by Week 1 and 160% by Week 9, and samples stored at -20 degrees C were 14% higher than samples stored at -80 degrees C (P < 0.0001). Experiment 4 assessed the effects of storage of whole blood samples (n = 15 blood samples collected for serum) at 4 degrees C for 0-7 days. Serum URN and GLB decreased (P < 0.0001), and TP and AMN increased (0.0001 < P < 0.02) with increasing refrigeration time. Standardized analytical procedures must be followed to obtain reliable nitrogenous metabolite measurements.

引用

页码：159 / 166

页数：8

共 25 条

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