MAMMALIAN DNA NUCLEOTIDE EXCISION-REPAIR RECONSTITUTED WITH PURIFIED PROTEIN-COMPONENTS

被引：751
作者：
ABOUSSEKHRA, A
BIGGERSTAFF, M
SHIVJI, MKK
VILPO, JA
MONCOLLIN, V
PODUST, VN
PROTIC, M
HUBSCHER, U
EGLY, JM
WOOD, RD
机构：
[1] IMPERIAL CANC RES FUND,CLARE HALL LABS,S MIMMS EN6 3LD,HERTS,ENGLAND
[2] INST GENET & BIOL MOLEC & CELLULAIRE,F-67404 ILLKIRCH GRAFFENS,FRANCE
[3] UNIV ZURICH IRCHEL,DEPT VET BIOCHEM,CH-8057 ZURICH,SWITZERLAND
[4] NICHHD,DNA REPLICAT REPAIR & MUTAGENESIS,BETHESDA,MD 20892
[5] TAMPERE UNIV HOSP,DEPT CLIN CHEM,SF-33521 TAMPERE,FINLAND
来源：
CELL | 1995年 / 80卷 / 06期
基金：
芬兰科学院; 美国国家卫生研究院;
关键词：
D O I：
10.1016/0092-8674(95)90289-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nucleotide excision repair is the principal way by which human cells remove UV damage from DNA. Human cell extracts were fractionated to locate active components, including xeroderma pigmentosum (XP) and ERCC factors. The incision reaction was then reconstituted with the purified proteins RPA, XPA, TFIIH (containing XPB and XPD), XPC, UV-DDB, XPG, partially purified ERCC1/XPF complex, and a factor designated IF7. UV-DDB (related to XPE protein) stimulated repair but was not essential. ERCC1- and XPF-correcting activity copurified with an ERCC1-binding polypeptide of 110 kDa that was absent in XP-F cell extract. Complete repair synthesis was achieved by combining these factors with DNA polymerase epsilon, RFC, PCNA, and DNA ligase I. The reconstituted core reaction requires about 30 polypeptides.
引用
收藏
页码：859 / 868
页数：10
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