CRYSTALLIZATION OF THE HYBRID BACILLUS (1-3,1-4)-BETA-GLUCANASE H(A16-M)

被引：7

作者：

KEITEL, T

GRANZIN, J

SIMON, O

BORRISS, R

THOMSEN, KK

WESSNER, H

HOHNE, W

HEINEMANN, U

机构：

[1] FREE UNIV BERLIN,INST KRISTALLOG,TAKUSTR 6,W-1000 BERLIN 33,GERMANY

[2] HUMBOLDT UNIV,BEREICH MIKROBIOL,SEKT NAHRUNGSGUTERWIRTSCHAFT & LEBENSMITTELTECHNOL,W-1017 BERLIN,GERMANY

[3] CARLSBERG LAB,DEPT PHYSIOL,DK-2500 COPENHAGEN,DENMARK

[4] HUMBOLDT UNIV,INST BIOCHEM,O-1040 BERLIN,GERMANY

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 218卷 / 04期

关键词：

D O I：

10.1016/0022-2836(91)90259-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extremely thermostable hybrid Bacillus (1-3, 1-4)-β-glucanase H(A16-M) has been crystallized with polyethylene glycol by vapour diffusion. The single crystals diffract to a resolution of 2.2 Å. The protein crystallizes in the orthorhombic space group P212121 with a = 70.22(7) A ̊, b = 72.56(1) A ̊, c = 49.97(1) A ̊, and has one molecule per asymmetric unit. © 1991.

引用

页码：703 / 704

页数：2