PHOTOAFFINITY-LABELING OF ANDROGEN RECEPTORS WITH 17-BETA-HYDROXY-17ALPHA-[H-3]METHYL-4,9,11-ESTRATRIEN-3-ONE

被引：26

作者：

BRINKMANN, AO

KUIPER, GGJM

DEBOER, W

MULDER, E

VANDERMOLEN, HJ

机构：

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1985年 / 126卷 / 01期

关键词：

D O I：

10.1016/0006-291X(85)90586-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthetic androgen 17.beta.-hydroxy-17.alpha.-[3H]methyl-4,9,11-estratrien-3-one (R1881 [methyltrienolone]) was used as a photoaffinity label to characterize androgen receptors in calf uterus and rat prostate. Polyacrylamide gel electrophoresis, under denaturing conditions, showed that the DNA-binding form of the androgen receptor in calf uterus cytosol is a protein with a molecular mass of 98 kD [kilodalton]. In rat prostate cytosol, an androgen receptor with a molecular mass of 46 kD could be photoaffinity labeled with R1881. The photoaffinity labeling procedure described here provides a method for studying the hormone binding domain of androgen receptors in partial purified preparations.

引用

页码：163 / 169

页数：7

共 22 条

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