Effect of cytochalasin B on 3-O-[C-14]-methyl-D-glucose or D-[U-C-14]glucose handling by BRIN-BD11 cells

被引：0

作者：

Cetik, Sibel ^{[1
]}

Rzajeva, Aigun ^{[1
]}

Malaisse, Willy J. ^{[1
]}

Sener, Abdullah ^{[1
]}

机构：

[1] Free Univ Brussels, Lab Expt Hormonol, 808 Route Lennik, B-1070 Brussels, Belgium

来源：

BIOMEDICAL REPORTS | 2014年 / 2卷 / 04期

关键词：

insulin-producing BRIN-BD11 cells; 3-O-[C-14]-methyl-D-glucoseorD-[U-C-14]glucose handling; cytochalasin B;

D O I：

10.3892/br.2014.269

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The present study aimed to investigate the effects of cytochalasin B ( 20 mu M) on the uptake of 3-O-[C-14]-methyl-D-glucose or D-[U-C-14]glucose (8.3 mM each) by BRIN-BD11 cells. Taking into account the distribution space of tritiated water ((HOH)-H-3), which was unexpectedly increased shortly after exposure of the cells to cytochalasin B and then progressively returned to its control values, and that of L-[1-C-14]glucose, used as an extracellular marker, it was demonstrated that cytochalasin B caused a modest, but significant inhibition of the uptake of D-glucose and its non-metabolized analog by the BRIN-BD11 cells. These findings resemble those observed in acinar or ductal cells of the rat submaxillary gland and displayed a relative magnitude comparable to that found for the inhibition of D-glucose metabolism by cytochalasin B in purified pancreatic islet B cells. These findings reinforce the view that the primary site of action of cytochalasin B is located at the level of the plasma membrane.

引用

页码：513 / 516

页数：4