ALTERATION OF THE EXPRESSION OF HUMAN ESTROGEN-RECEPTOR GENE BY DISTAMYCIN

被引：23
作者：
BIANCHI, N
PASSADORE, M
FERIOTTO, G
MISCHIATI, C
GAMBARI, R
PIVA, R
机构：
[1] UNIV FERRARA,DIPARTMENTO BIOCHIM & BIOL MOLEC,I-44100 FERRARA,ITALY
[2] UNIV FERRARA,CTR INTERDIPARTIMENTALE BIOTECNOL,I-44100 FERRARA,ITALY
来源：
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1995年 / 54卷 / 5-6期
关键词：
D O I：
10.1016/0960-0760(95)00133-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of distamycin on the expression of the estrogen receptor gene were determined in the MCF7 human breast cancer cell line. Estrogen receptor (ER) RNA transcripts were analyzed by Northern blotting and RT-PCR using specific oligonucleotides for the 5' upstream region and for ER cDNA. After ex vivo distamycin treatment of the cells the expression of the canonical ER mRNA isoform of 6.3 kb is strongly inhibited, without appreciable alteration of the accumulation of 5' upstream ER mRNA isoforms. These results suggest that distamycin alters the transcriptional activity of the ER gene causing a change in the ratio between the canonical transcript and other isoforms containing 5' upstream regions.
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页码：211 / 215
页数：5
相关论文
共 25 条
[1]   17-BETA-ESTRADIOL REGULATES AND V-HA-RAS TRANSFECTION CONSTITUTIVELY ENHANCES MCF7 BREAST-CANCER CELL-INTERACTIONS WITH BASEMENT-MEMBRANE [J].
ALBINI, A ;
GRAF, J ;
KITTEN, GT ;
KLEINMAN, HK ;
MARTIN, GR ;
VEILLETTE, A ;
LIPPMAN, ME .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8182-8186
[2]   ESTROGEN-RECEPTOR STATUS - IMPORTANT VARIABLE IN PREDICTING RESPONSE TO ENDOCRINE THERAPY IN METASTATIC BREAST-CANCER [J].
ALLEGRA, JC ;
LIPPMAN, ME ;
THOMPSON, EB ;
SIMON, R ;
BARLOCK, A ;
GREEN, L ;
HUFF, KK ;
DO, HMT ;
AITKEN, SC ;
WARREN, R .
EUROPEAN JOURNAL OF CANCER, 1980, 16 (03) :323-331
[3]   DISTAMYCINS INHIBIT THE BINDING OF OTF-1 AND NFE-1 TRANSFACTORS TO THEIR CONSERVED DNA ELEMENTS [J].
BROGGINI, M ;
PONTI, M ;
OTTOLENGHI, S ;
DINCALCI, M ;
MONGELLI, N ;
MANTOVANI, R .
NUCLEIC ACIDS RESEARCH, 1989, 17 (03) :1051-1059
[4]   DESIGN OF SEQUENCE-SPECIFIC DNA-BINDING MOLECULES [J].
DERVAN, PB .
SCIENCE, 1986, 232 (4749) :464-471
[5]   DISTAMYCIN-INDUCED INHIBITION OF HOMEODOMAIN DNA COMPLEXES [J].
DORN, A ;
AFFOLTER, M ;
MULLER, M ;
GEHRING, WJ ;
LEUPIN, W .
EMBO JOURNAL, 1992, 11 (01) :279-286
[6]  
EVANS RM, 1989, SCIENCE, V240, P889
[7]   BINDING OF EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-1 TO DNA - INHIBITION BY DISTAMYCIN AND 2 NOVEL DISTAMYCIN ANALOGS [J].
FERIOTTO, G ;
CIUCCI, A ;
MISCHIATI, C ;
ANIMATI, F ;
LOMBARDI, P ;
GIACOMINI, P ;
ARCAMONE, F ;
GAMBARI, R .
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1994, 267 (02) :143-149
[8]   SEQUENCE-SPECIFIC RECOGNITION OF THE HIV-1 LONG TERMINAL REPEAT BY DISTAMYCIN - A DNAASE-I FOOTPRINTING STUDY [J].
FERIOTTO, G ;
MISCHIATI, C ;
GAMBARI, R .
BIOCHEMICAL JOURNAL, 1994, 299 :451-458
[9]  
FUQUA SAW, 1992, CANCER RES, V52, P483
[10]   DNA-BINDING ACTIVITY AND BIOLOGICAL EFFECTS OF AROMATIC POLYAMIDINES [J].
GAMBARI, R ;
NASTRUZZI, C .
BIOCHEMICAL PHARMACOLOGY, 1994, 47 (04) :599-610
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