DETECTION OF MESSENGER-RNA AND LOW-ABUNDANCE HETERONUCLEAR RNA WITH SINGLE-STRANDED-DNA PROBES PRODUCED BY AMPLIFIED PRIMER EXTENSION LABELING

被引：25

作者：

BROOKS, PJ ^{[1
]}

KAPLITT, MG ^{[1
]}

KLEOPOULOS, SP ^{[1
]}

FUNABASHI, T ^{[1
]}

MOBBS, CV ^{[1
]}

PFAFF, DW ^{[1
]}

机构：

[1] ROCKEFELLER UNIV, NEUROBIOL & BEHAV LAB, NEW YORK, NY 10021 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1993年 / 41卷 / 12期

关键词：

INTRON; OXYTOCIN; PREPROENKEPHALIN; RNA PROCESSING;

D O I：

10.1177/41.12.8245424

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe a procedure for detection of low-abundance cellular RNAs by in situ hybridization histochemistry, using single-stranded DNA probes produced by amplified primer extension labeling with Tag polymerase. We have used this approach to detect a number of high- and low-abundance RNA species and have found it to be a simple and reproducible method of obtaining sensitive probes for in situ hybridization studies. For example, DNA probes generated by amplified primer extension labeling can detect low-abundance heteronuclear RNAs in individual neurons. Since this procedure does not involve recombinant DNA technology or microbiological facilities, it should prove useful to a wide variety of investigators studying the regulation of gene expression at the cellular level.

引用

页码：1761 / 1766

页数：6

共 27 条

[1] THE LOCATION OF ULTRABITHORAX TRANSCRIPTS IN DROSOPHILA TISSUE-SECTIONS [J].