BIOSYNTHESIS AND SECRETION OF MUCIN-RELATED PRODUCTS IN HS746T GASTRIC-CANCER CELLS

被引：1

作者：

SANO, S ^{[1
]}

OKAZAKI, K ^{[1
]}

YAMAMOTO, Y ^{[1
]}

机构：

[1] KOCHI MED SCH,DEPT INTERNAL MED 1,NANKOKU,KOCHI 783,JAPAN

来源：

JOURNAL OF GASTROENTEROLOGY | 1994年 / 29卷 / 05期

关键词：

MUCIN; N-GLYCAN; GASTRIC ADENOCARCINOMA;

D O I：

10.1007/BF02365437

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We have previously studied the biosynthesis and secretion of mucin in the normal human stomach and reported that the tetramer of the 60-kDa subunit of mucin core protein was synthesized and highly glycosylated, and that only high molecular weight mucin was secreted. In this study, we investigated the mucin-related products of a gastric cancer cell line (Hs746T). Analysis of intracellular and extracellular products immunoprecipitated with an anti-apomucin monoclonal antibody revealed that a 110-kDa protein, the dimer of the 55-kDa subunit, was synthesized and secreted. Tunicamycin treatment inhibited the secretion of the 110-kDa protein. These findings suggest that N-glycosylation may be involved in the secretory mechanism of the mucin-related product.

引用

页码：569 / 576

页数：8

共 55 条

[1]

Okazaki K., Sano S., Yamamoto Y., Biosynthesis of gastric mucin in the human, Gastroenterology, 102, (1992)

[2]

Neutra, Forstner J.F., Gastrointestinal mucus: Synthesis, secretion, and function, Physiology of the gastrointestinal tract. Vol 1, pp. 975-1009, (1987)

[3]

Koprowski H., Herlyn M., Steplewski Z., Specific antigen in serum of patients with colon carcinoma, Science, 212, pp. 53-55, (1981)

[4]

Klug T.L., Bast R.C., Nilloff J.M., Et al., A monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 125) associated with human epithelial ovarian carcinomas, Cancer Res, 44, pp. 1048-1053, (1984)

[5]

Holmgren J., Lindholm L., Persson B., Et al., Detection by monoclonal antibody of carbohydrate antigen CA50 in serum of patients with carcinoma, BMJ, 288, pp. 1479-1482, (1984)

[6]

Metzgar R.S., Gaillard M.T., Levine S.J., Et al., Antigens of human pancreatic adenocarcinoma cells defined by murine monoclonal antibodies, Cancer Res, 42, pp. 601-608, (1982)

[7]

Metzgar R.S., Rodriguez N., Finn O.J., Et al., Detectoon of pancreatic cancer-associated antigen (Du-Pan-II antigen) in serum and ascites of patients with adenocarcinoma, Proc Natl Acad Sci USA, 81, pp. 5242-5246, (1986)

[8]

Hayes D.F., Sekine H., Ohno T., Et al., Use of a murine monoclonal antibody for detection of circulating plasma DF3 antigen levels in breast cancer patients, J Clin Invest, 75, pp. 1671-1678, (1985)

[9]

Ban T., Imai K., Yachi A., Immunohistochemical characterization of a novel pancreatic cancer-associated antigen MUSE11, Cancer Res, 49, pp. 7141-7146, (1989)

[10]

Morita M., Okazaki K., Tamura S., Et al., Measurement of serum MP-A11 in patients with cancer of digestive organs, Jpn J Gastroenterology, 84, (1987)

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