PYRIDINE NUCLEOTIDE-DEPENDENT CHANGES IN THE PHASE-PARTITIONING BEHAVIOR OF NADP-LINKED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PEA-CHLOROPLASTS

被引：1

作者：

ANDERSON, LE

LI, D

PACOLD, MM

机构：

[1] Department of Biological Sciences, University of Illinois at Chicago, Chicago

来源：

PLANT SCIENCE | 1994年 / 99卷 / 01期

基金：

美国国家科学基金会;

关键词：

CHLOROPLAST GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; PHASE PARTITIONING; AGGREGATION; CONFORMATIONAL CHANGE; NAD; NADP;

D O I：

10.1016/0168-9452(94)90118-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Addition of NAD(H) or NADP(H) to an aqueous two-phase polyethylene glycol-dextran system affects the partitioning of purified pea (Pisum sativum L.) chloroplast glyceraldehyde-3-P dehydrogenase. The results indicate that the nature of the bound pyridine nucleotide affects the conformation and the state of aggregation of the enzyme, as suggested earlier by size exclusion (R. Cerff, fur. J. Biochem., 82 (1978) 45-53) and sucrose density gradient centrifugation (P. Pupillo and G. Giuliani Picccari, fur. J. Biochem., 51 (1975) 475-482) experiments.

引用

页码：39 / 42

页数：4

共 46 条

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[23] EFFECT OF CHLORAMPHENICOL ON DEVELOPMENT OF PROPLASTIDS IN EUGLENA-GRACILIS .1. SYNTHESIS OF RIBULOSEDIPHOSPHATE CARBOXYLASE, NADP-LINKED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND AMINOLEVULINATE DEHYDRATASE
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[24] EFFECT OF X-RAYS AND INHIBITORS OF PROTEIN-SYNTHESIS ON SYNTHESIS OF CHLOROPHYLL AND NADP-LINKED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN GREENING EUGLENA-GRACILIS
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PROTEIN EXPRESSION AND PURIFICATION, 2002, 25 (03) : 519 - 526
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WAGNER, E
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[29] Chlamydomonas reinhardtii NADP-linked glyceraldehyde-3-phosphate dehydrogenase contains the cysteine residues identified as potentially domain-locking in the higher plant enzyme and is light activated
Li A.D.
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Kersanach R.
Anderson L.E.
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Stevens, FJ
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Kersanach, R
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