PYRIDINE NUCLEOTIDE-DEPENDENT CHANGES IN THE PHASE-PARTITIONING BEHAVIOR OF NADP-LINKED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PEA-CHLOROPLASTS

Addition of NAD(H) or NADP(H) to an aqueous two-phase polyethylene glycol-dextran system affects the partitioning of purified pea (Pisum sativum L.) chloroplast glyceraldehyde-3-P dehydrogenase. The results indicate that the nature of the bound pyridine nucleotide affects the conformation and the state of aggregation of the enzyme, as suggested earlier by size exclusion (R. Cerff, fur. J. Biochem., 82 (1978) 45-53) and sucrose density gradient centrifugation (P. Pupillo and G. Giuliani Picccari, fur. J. Biochem., 51 (1975) 475-482) experiments.