AGE ESTIMATION USING A MODIFIED HPLC DETERMINATION OF RATIO OF ASPARTIC-ACID IN DENTIN

被引：41

作者：

FU, SJ

FAN, CC

SONG, HW

WEI, FQ

机构：

[1] CHINA MED UNIV, DEPT FORENS ANTHROPOL, SHENYANG 110001, PEOPLES R CHINA

[2] LIAONING INST BASIC MED SCI, SHENYANG 110005, PEOPLES R CHINA

来源：

FORENSIC SCIENCE INTERNATIONAL | 1995年 / 73卷 / 01期

关键词：

AGE ESTIMATION; HPLC; ASPARTIC ACID; DENTIN;

D O I：

10.1016/0379-0738(95)01709-7

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine. The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector. A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used, The sensitivity of detection was 1 pmol and high resolution (Rs = 1.5) was reached, We determined the D/L ratio of aspartic acid in dentin of 28 first premolars, The correlation value between the D/L ratio of aspartic acid and actual age was 0.9887; errors of +/-1 year accounted for 46.4%, and no error exceeded 5 years.

引用

页码：35 / 40

页数：6

共 11 条

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[Anonymous], 1968, CARNEGIE I WASH YR B

[2] AN IMPROVED METHOD FOR AGE AT DEATH DETERMINATION FROM THE MEASUREMENT OF D-ASPARTIC ACID IN DENTAL COLLAGEN [J].