RAPID METHOD FOR ISOLATION AND STAINING OF BACTERIAL LIPOPOLYSACCHARIDE

被引：19

作者：

ALHENDY, A

TOIVANEN, P

SKURNIK, M

机构：

[1] Department of Medical Microbiology, Turku University

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 35卷 / 04期

关键词：

D O I：

10.1111/j.1348-0421.1991.tb01562.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem(TM) both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.

引用

页码：331 / 333

页数：3

共 5 条

[1] MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS [J].