EFFECTS OF MAGNESIUM AND IRON ON LIPID-PEROXIDATION IN CULTURED-HEPATOCYTES

被引：40
作者：
GUNTHER, T
VORMANN, J
HOLLRIEGL, V
机构：
[1] Institute of Molecular Biology and Biochemistry, Free University of Berlin, Berlin, D-14195
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 1995年 / 144卷 / 02期
关键词：
MAGNESIUM; IRON; LIPID PEROXIDATION; HEPATOCYTE;
D O I：
10.1007/BF00944393
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
In primary cultures of rat hepatocytes, the effects of extracellular Mg2+ and Fe on lipid peroxidation (LPO) as measured by means of malondialdehyde (MDA) formation were investigated. Incubation of hepatocytes at decreasing extracellular Mg2+ concentration enhanced LPO, depending on extracellular Fe. About 96% of MDA accumulated in the culture medium. Addition of desferrioxamine prevented LPO. Additionally, the formation of oxygen free radicals was determined by fluorescence reduction of cis-parinaric acid. With this method, an immediate decay of fluorescence was found after addition of Fe2+. Fluorescence reduction was completely prevented by desferrioxamine, indicating the function of extracellular Fe. This mechanism may operate additionally to the increase in intracellular Fe and intracellular formation of oxygen free radicals during Mg deficiency in vivo.
引用
收藏
页码：141 / 145
页数：5
相关论文
共 27 条
  • [1] HEPATIC MITOCHONDRIAL MALONDIALDEHYDE METABOLISM IN RATS WITH CHRONIC IRON OVERLOAD
    BRITTON, RS
    ONEILL, R
    BACON, BR
    [J]. HEPATOLOGY, 1990, 11 (01) : 93 - 97
  • [2] A REVIEW OF RECENT STUDIES ON THE METABOLISM OF EXOGENOUS AND ENDOGENOUS MALONDIALDEHYDE
    DRAPER, HH
    HADLEY, M
    [J]. XENOBIOTICA, 1990, 20 (09) : 901 - 907
  • [3] ELIN RJ, 1980, MAGNESIUM HLTH DISEA, P125
  • [4] GUNTHER T, 1981, MAGNESIUM-B, V3, P91
  • [5] GUNTHER T, 1994, MAGNESIUM-B, V16, P38
  • [6] GUNTHER T, 1992, MAGNESIUM-B, V14, P57
  • [7] GUNTHER T, 1992, MAGNESIUM-B, V14, P81
  • [8] MATERNAL AND FETAL IRON ACCUMULATION IN ZN-DEFICIENT AND SALICYLATE-TREATED RATS
    GUNTHER, T
    GOSSRAU, R
    VORMANN, J
    HOLLRIEGL, V
    GRAF, R
    [J]. BIOLOGICAL TRACE ELEMENT RESEARCH, 1988, 18 : 49 - 58
  • [9] CHARACTERIZATION OF THIOBARBITURIC ACID REACTIVITY IN HUMAN-PLASMA AND URINE
    GUTTERIDGE, JMC
    TICKNER, TR
    [J]. ANALYTICAL BIOCHEMISTRY, 1978, 91 (01) : 250 - 257
  • [10] THE MEASUREMENT AND MECHANISM OF LIPID-PEROXIDATION IN BIOLOGICAL-SYSTEMS
    GUTTERIDGE, JMC
    HALLIWELL, B
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (04) : 129 - 135
123