TRICHOTHECENE PRODUCTION BY FUSARIUM SPP ISOLATED FROM MANITOBA GRAIN

被引：49
作者：
ABRAMSON, D
CLEAR, RM
SMITH, DM
机构：
[1] Research Station, Agriculture Canada, Winnipeg, MB
来源：
CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE | 1993年 / 15卷 / 03期
关键词：
D O I：
10.1080/07060669309500815
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Thirty-five isolates of common Fusarium species from Manitoba wheat, and one from Manitoba corn, were tested for trichothecene production in liquid cultures. The Fusarium species were: F. acuminatum, F. avenaceum, F. crookwellense, F. culmorum, F. equiseti, F. graminearum, F. poae, and F. sporotrichioides. The culture media were solvent-extracted, and the purified residues assayed for selected trichothecene mycotoxins by gas chromatography/mass spectrometry. Cultures of F. sporotrichioides produced T-2 and HT-2 toxins, diacetoxyscirpenol (DAS), neosolaniol (NSL), 15-acetyldeoxynivalenol (15ADON), fusarenone-X (FX), deoxynivalenol (DON), and nivalenol (NIV). These trichothecenes, with the exception of NSL, were also present in cultures of F. graminearum and F. poae. Fusarium culmorum cultures produced HT-2 toxin, 15ADON, FX, DON, and NIV; F. equiseti cultures produced similar trichothecenes, but with DAS present instead of NIV. A single isolate of F. crookwellense from corn produced HT-2 toxin, 15ADON, FX, and DON; F. avenaceum cultures produced only 15ADON and DON. In F. acuminatum cultures, only T-2 toxin, FX, and DON were detected. F. graminearum, F. poae and F. sporotrichioides produced the greatest amount and the greatest number of trichothecene mycotoxins. Maximum levels of 124.6 mug T-2 toxin/mL culture were observed for one isolate of F. sporotrichioides.
引用
收藏
页码：147 / 152
页数：6
相关论文
共 22 条
  • [1] Abramson D., Clear R.M., Nowicki T.W., Fusarium species and trichothecene mycotoxins in suspect samples of 1985 manitoba wheat, Can. J. Plant Sci, 67, pp. 611-619, (1987)
  • [2] Abramson D., Thorsteinson T., Forest D., Chromatography of mycotoxins on precoated reverse-phase thin-layer plates, Arch. Environ. Contam, 18, pp. 327-330, (1989)
  • [3] Bamburg J.R., Strong F.M., Mycotoxins of the trichothecane family produced by fusarium tricinctum and trichoderma lignorum, Phytochemistry, 8, pp. 2405-2410, (1969)
  • [4] Clear R.M., Patrick S.K., Fusarium species isolated from wheat samples containing tombstone (Scab) kernels from ontario, manitoba, and saskatchewan, Can. J. Plant Sci, 70, pp. 1057-1069, (1990)
  • [5] Faifer G.C., De Miguel M.S., Godoy H.M., Patterns of mycotoxin production by fusarium graminearum isolated from argentine wheat, Mycopathologia, 109, pp. 165-170, (1990)
  • [6] Forsell J.H., Jensen R., Tai J.-H., Witt M., Lin W.S., Petska J.J., Comparison of the Acute Toxicities of Deoxynivalenol (Vomitoxin) and 15-Acetyldeoxynivalenol in the B6C3F, Mouse, 25, pp. 155-162, (1987)
  • [7] Greenhalgh R., Levandier D., Adams W., Miller J.D., Blackwell B.A., McAlees A.J., Taylor A., Production and characterization of deoxynivalenol and other secondary metabolites of fusarium culmorum (Cmi 14764, hlx 1503), J. Agric. Food Chem, 34, pp. 98-102, (1986)
  • [8] Greenhalgh R., Blackwell B.A., Savard M.E., Miller J.D., Taylor A., Secondary metabolites of fusarium sporotrichioides daom 165006 in liquid culture, J. Agric. Food Chem, 36, pp. 216-219, (1988)
  • [9] Gregory K.F., Allen O.N., Riker A.J., Peterson W.H., Antibiotics as Agents for the Control of Certain Damping- off Fungi, 39, pp. 405-415, (1952)
  • [10] Ichinoe M., Kurata H., Sugiura Y., Ueno Y., Chemotaxonomy of gibberella zeae with special reference to production of trichothecenes and zearalenone, Appl. Env. Microbiol, 46, pp. 1364-1369, (1983)
123