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EXPRESSION OF POKEWEED (PHYTOLACCA-AMERICANA) ANTIVIRAL PROTEIN CDNA IN ESCHERICHIA-COLI AND ITS ANTIVIRAL ACTIVITY
被引:11
作者
:
CHEN, ZC
论文数:
0
引用数:
0
h-index:
0
机构:
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
CHEN, ZC
[
1
]
ANTONIW, JF
论文数:
0
引用数:
0
h-index:
0
机构:
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
ANTONIW, JF
[
1
]
LIN, Q
论文数:
0
引用数:
0
h-index:
0
机构:
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
LIN, Q
[
1
]
WHITE, RF
论文数:
0
引用数:
0
h-index:
0
机构:
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
WHITE, RF
[
1
]
机构
:
[1]
AFRC,INST ARABLE CROPS RES,DEPT PLANT PATHOL,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
来源
:
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
|
1993年
/ 42卷
/ 04期
关键词
:
D O I
:
10.1006/pmpp.1993.1022
中图分类号
:
Q94 [植物学];
学科分类号
:
071001 ;
摘要
:
Two DNA constructs encoding the pokeweed antiviral protein (PAP), one encoding PAP and its signal peptide and the other encoding PAP, were made using polymerase chain reactions (PCR), inserted into the expression vector pKK233-2 and transformed into Escherichia coli JM109 cells. The transformants containing the gene encoding PAP with its signal peptide were able to grow and produce the PAP protein. Two protein bands with molecular weights of 30 kDa and 33 kDa, corresponding to PAP without and with its signal peptide, were detected using monospecific PAP antiserum in Western blots. This suggested that the signal peptide of PAP could be recognized in E. coli but not as efficiently as in pokeweed plant cells. The concentration of PAP expressed in E. coli was low, with only 0·37 mg of PAP produced from II of bacterial culture in LB medium, constituting 0·13% of the total bacterial protein. A slightly higher yield of PAP, 0·9 mg PAP I-1of culture which accounts for 0·16% of total protein, was achieved by culturing in M9 medium. Purified PAP depurinated the rRNA of E. coli as well as yeast and plants and probably inactivates the ribosomes in the same way as other ribosome-inactivating proteins (RIPs). This strong inhibition of E. coli ribosomes may explain the difficulty of expressing the PAP gene in E. coli. The PAP produced in E. coli was able to inhibit infection of tobacco by TMV. © 1993 Academic Press, Inc.
引用
收藏
页码:237 / 247
页数:11
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共 24 条
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[J].
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[2]
PURIFICATION AND PARTIAL CHARACTERIZATION OF ANOTHER FORM OF THE ANTIVIRAL PROTEIN FROM THE SEEDS OF PHYTOLACCA-AMERICANA L (POKEWEED)
[J].
BARBIERI, L
论文数:
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;
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[J].
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论文数:
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h-index:
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机构:
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BEREMAND, PD
;
HANNAPEL, DJ
论文数:
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引用数:
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h-index:
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机构:
PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907
HANNAPEL, DJ
;
GUERRA, DJ
论文数:
0
引用数:
0
h-index:
0
机构:
PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907
GUERRA, DJ
;
KUHN, DN
论文数:
0
引用数:
0
h-index:
0
机构:
PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907
KUHN, DN
;
OHLROGGE, JB
论文数:
0
引用数:
0
h-index:
0
机构:
PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907
OHLROGGE, JB
.
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(01)
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[5]
INSENSITIVITY OF ARCHAEBACTERIAL RIBOSOMES TO PROTEIN-SYNTHESIS INHIBITORS - EVOLUTIONARY IMPLICATIONS
[J].
CAMMARANO, P
论文数:
0
引用数:
0
h-index:
0
机构:
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CAMMARANO, P
;
TEICHNER, A
论文数:
0
引用数:
0
h-index:
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机构:
CNR,IST CHIM MOLEC INTERESSE BIOL,NAPLES,ITALY
TEICHNER, A
;
LONDEI, P
论文数:
0
引用数:
0
h-index:
0
机构:
CNR,IST CHIM MOLEC INTERESSE BIOL,NAPLES,ITALY
LONDEI, P
;
ACCA, M
论文数:
0
引用数:
0
h-index:
0
机构:
CNR,IST CHIM MOLEC INTERESSE BIOL,NAPLES,ITALY
ACCA, M
;
NICOLAUS, B
论文数:
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引用数:
0
h-index:
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机构:
CNR,IST CHIM MOLEC INTERESSE BIOL,NAPLES,ITALY
NICOLAUS, B
;
SANZ, JL
论文数:
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引用数:
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h-index:
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机构:
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SANZ, JL
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机构:
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AMILS, R
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(03)
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[6]
EFFECT OF POKEWEED ANTIVIRAL PROTEIN (PAP) ON THE INFECTION OF PLANT-VIRUSES
[J].
CHEN, ZC
论文数:
0
引用数:
0
h-index:
0
机构:
Department of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire
CHEN, ZC
;
WHITE, RF
论文数:
0
引用数:
0
h-index:
0
机构:
Department of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire
WHITE, RF
;
ANTONIW, JF
论文数:
0
引用数:
0
h-index:
0
机构:
Department of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire
ANTONIW, JF
;
LIN, Q
论文数:
0
引用数:
0
h-index:
0
机构:
Department of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire
LIN, Q
.
PLANT PATHOLOGY,
1991,
40
(04)
:612
-620
[7]
DUGGAR B. M., 1925, Ann. Mo. Bot. Gdn., V12, P359, DOI 10.2307/2394061
[8]
SYNTHESIS AND PROCESSING OF THE PLANT PROTEIN THAUMATIN IN YEAST
[J].
EDENS, L
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EDENS, L
;
BOM, I
论文数:
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h-index:
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BOM, I
;
LEDEBOER, AM
论文数:
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引用数:
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h-index:
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LEDEBOER, AM
;
MAAT, J
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MAAT, J
;
TOONEN, MY
论文数:
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TOONEN, MY
;
VISSER, C
论文数:
0
引用数:
0
h-index:
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VISSER, C
;
VERRIPS, CT
论文数:
0
引用数:
0
h-index:
0
VERRIPS, CT
.
CELL,
1984,
37
(02)
:629
-633
[9]
THE SITE OF ACTION OF 6 DIFFERENT RIBOSOME-INACTIVATING PROTEINS FROM PLANTS ON EUKARYOTIC RIBOSOMES - THE RNA N-GLYCOSIDASE ACTIVITY OF THE PROTEINS
[J].
ENDO, Y
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV TUMOR IMMUNOL,44 BINNEY ST,BOSTON,MA 02115
ENDO, Y
;
TSURUGI, K
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV TUMOR IMMUNOL,44 BINNEY ST,BOSTON,MA 02115
TSURUGI, K
;
LAMBERT, JM
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV TUMOR IMMUNOL,44 BINNEY ST,BOSTON,MA 02115
LAMBERT, JM
.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,
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150
(03)
:1032
-1036
[10]
REGULATION OF COLIPHAGE-T3 AND COLIPHAGE-T7 RNA-POLYMERASES BY THE LAC REPRESSOR-OPERATOR SYSTEM
[J].
GIORDANO, TJ
论文数:
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引用数:
0
h-index:
0
机构:
SUNY HLTH SCI CTR,MORSE INST MOLEC BIOL & GENET,DEPT MICROBIOL & IMMUNOL,BOX 44,450 CLARKSON AVE,BROOKLYN,NY 11203
GIORDANO, TJ
;
DEUSCHLE, U
论文数:
0
引用数:
0
h-index:
0
机构:
SUNY HLTH SCI CTR,MORSE INST MOLEC BIOL & GENET,DEPT MICROBIOL & IMMUNOL,BOX 44,450 CLARKSON AVE,BROOKLYN,NY 11203
DEUSCHLE, U
;
BUJARD, H
论文数:
0
引用数:
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h-index:
0
机构:
SUNY HLTH SCI CTR,MORSE INST MOLEC BIOL & GENET,DEPT MICROBIOL & IMMUNOL,BOX 44,450 CLARKSON AVE,BROOKLYN,NY 11203
BUJARD, H
;
MCALLISTER, WT
论文数:
0
引用数:
0
h-index:
0
机构:
SUNY HLTH SCI CTR,MORSE INST MOLEC BIOL & GENET,DEPT MICROBIOL & IMMUNOL,BOX 44,450 CLARKSON AVE,BROOKLYN,NY 11203
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.
GENE,
1989,
84
(02)
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