IDENTIFICATION OF MOLECULAR MARKERS LINKED TO THE AGROPYRON ELONGATUM-DERIVED LEAF RUST RESISTANCE GENE LR24 IN WHEAT

被引：145

作者：

SCHACHERMAYR, GM ^{[1
]}

MESSMER, MM ^{[1
]}

FEUILLET, C ^{[1
]}

WINZELER, H ^{[1
]}

WINZELER, M ^{[1
]}

KELLER, B ^{[1
]}

机构：

[1] SWISS FED RES STN AGRON,DEPT PLANT BREEDING,CH-8046 ZURICH,SWITZERLAND

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 7-8期

关键词：

LEAF RUST; RFLP; RAPD; WHEAT; AGROPYRON ELONGATUM;

D O I：

10.1007/BF00222911

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The objective of this study was to identify molecular markers linked to the wheat leaf rust resistance gene Lr24 derived from Agropyron elongatum (3DL/3 Ag translocation). Two near isogenic lines (NILs), 'Arina' and Lr24/7* ''Arina'', were screened for polymorphism at the DNA level with 115 RFLP probes. Twenty-one of these probes map to the homoeologous group 3. In addition, 360 RAPD primers were tested on the NILs. Six RFLP probes showed polymorphism between the NILs, and 11 RAPD primers detected one additional band in the resistant NIL. The genetic linkage of the polymorphic markers with Lr24 was tested on a segregating F-2 population (150 plants) derived from a cross between the leaf rust resistant Lv24/7* ''Arina'' and the susceptible spelt (Triticum spelta) variety 'Oberkulmer'. All 6 RFLP markers were completely linked to Lr24: one was inherited as a codominant marker (PSR1205), one was in coupling phase (PSR1203) and 4 were in repulsion phase (PSR388, PSR904, PSR931, PSR1067) with Lr24. The localization of these probes on chromosome 3D was confirmed by nulli-tetrasomic analysis. Distorted,genotypic segregation was found for the codominant RFLP marker PSR1205. This distortion can be explained by the occurrence of hemizygous plants. One of the 11 RAPD markers (OPJ-09) also showed complete linkage to the Lr24 resistance gene. The polymorphic RAPD fragment was cloned and sequenced. Specific primers were synthesized, and they produced an amplification product only in the resistant plants. This specific marker allows a reliable and rapid screening of a large number of genotypes in practical breeding. Analysis of 6 additional lines containing Lr24 revealed that 3 lines have a smaller chromosomal segment of A. elongatum than lines derived from 'Agent', a commonly used gene donor for the Lr24 resistance gene.

引用

页码：982 / 990

页数：9

共 29 条

[1]

Baum M., Lagudah E.S., Appels R., Wide crosses in cereals, Annu Rev Plant Physiol Plant Mol Biol, 43, pp. 117-143, (1992)

[2]

Browder L.E., Probable genotype of some Triticum aestivum ‘Agent’ derivatives for reaction to Puccinia recondita f. sp. tritici, Crop Science, 13, pp. 203-206, (1973)

[3]

Devos K.M., Gale M.D., The use of random amplified polymorphic DNA markers in wheat, Theor Appl Genet, 84, pp. 567-572, (1992)

[4]

Devos K.M., Gale M.D., Extended genetic maps of the homoeologous group 3 chromosomes of wheat rye and barley, Theor Appl Genet, 85, pp. 649-652, (1993)

[5]

Devos K.M., Atkinson M.D., Chinoy C.N., Liu C.J., Gale M.D., RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye, Theor Appl Genet, 83, pp. 931-939, (1992)

[6]

Devos K.M., Millan T., Gale M.D., Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley, Theor Appl Genet, 85, pp. 784-792, (1993)

[7]

Graner A., Siedler H., Jahoor A., Herrmann R.G., Wenzel G., Assessment of the degree and type of restriction fragment length polymorphism in barley (Hordeum vulgare), Theor Appl Genet, 80, pp. 826-832, (1990)

[8]

Hartl L., Weiss H., Zeller F.J., Jahoor A., Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.), Theor Appl Genet, 86, pp. 959-963, (1993)

[9]

Knott D.R., The wheat rusts — breeding for resistance, (1989)

[10]

Kolmer J.A., Physiologic specialization of Puccinia recondita f. sp. tritici in Canada in 1991, Canadian Journal of Plant Pathology, 15, pp. 34-36, (1993)

← 1 2 3 →