Complete Genome Sequence of an Emerging Genotype of Tobacco Streak Virus in the United States

被引:7
作者
Padmanabhan, Chellappan [1 ]
Gao, Shan [2 ]
Li, Rugang [1 ]
Zhang, Shouan [3 ,4 ]
Fei, Zhangjun [2 ,5 ]
Ling, Kai-Shu [1 ]
机构
[1] ARS, Dept Agr, US Vegetable Lab, Charleston, SC 29414 USA
[2] Cornell Univ, Boyce Thompson Inst Plant Res, New York, NY 10021 USA
[3] Univ Florida, IFAS, Ctr Trop Res & Educ, Homestead, FL 33031 USA
[4] Univ Florida, Dept Plant Pathol, IFAS, Homestead, FL USA
[5] ARS, Dept Agr, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA
基金
美国食品与农业研究所;
关键词
D O I
10.1128/genomeA.01138-14
中图分类号
学科分类号
摘要
We report here the complete genome sequence of an emerging genotype of tobacco streak virus (TSV) infecting zucchini squash in Florida (TSV_FL13-07), obtained using deep sequencing of short RNAs (sRNAs) and validation by Sanger sequencing. TSV_FL13-07 shares only <90% sequence identity in all three genomic RNAs to several known U.S. isolates.
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共 4 条
[1]   A cost-effective method for Illumina small RNA-Seq library preparation using T4 RNA ligase 1 adenylated adapters [J].
Chen, Yun-Ru ;
Zheng, Yi ;
Liu, Bao ;
Zhong, Silin ;
Giovannoni, Jim ;
Fei, Zhangjun .
PLANT METHODS, 2012, 8
[2]   Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses [J].
Kreuze, Jan F. ;
Perez, Ana ;
Untiveros, Milton ;
Quispe, Dora ;
Fuentes, Segundo ;
Barker, Ian ;
Simon, Reinhard .
VIROLOGY, 2009, 388 (01) :1-7
[3]   Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate [J].
Li, Robert W. ;
Wu, Sitao ;
Baldwin, Ransom L. ;
Li, Weizhong ;
Li, Congjun .
PLOS ONE, 2012, 7 (01)
[4]   Complete Genome Sequence of a New Tobamovirus Naturally Infecting Tomatoes in Mexico [J].
Li, Rugang ;
Gao, Shan ;
Fei, Zhangjun ;
Ling, Kai-Shu .
GENOME ANNOUNCEMENTS, 2013, 1 (05)