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LOCAL COMPARISON OF THE GENOMES OF MYCOBACTERIUM-TUBERCULOSIS AND MYCOBACTERIUM-LEPRAE USING THE POLYMERASE CHAIN-REACTION
被引:0
作者:
PHILIPP, WJ
[1
]
COLE, ST
[1
]
机构:
[1] INST PASTEUR, UNITE GENET MOLEC BACTERIENNE, F-75724 PARIS 15, FRANCE
关键词:
MYCOBACTERIUM TUBERCULOSIS;
POLYMERASE CHAIN REACTION;
GENOME ORGANIZATION;
GENOME MAP;
MYCOBACTERIUM LEPRAE;
D O I:
暂无
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
To facilitate comparison of the genome maps of Mycobacterium tuberculosis and M. leprae, sequence data from the M. leprae sequencing project were used to design primers suitable for the amplification of short segments from conserved genes by the polymerase chain reaction. In most cases, both organisms yielded products of identical size that were then used as probes for a comparative genomic walk. The hybridization data often, but not always, revealed a similar organization of various regions of both genomes. This approach should be useful for systematic comparisons of (myco)bacterial genomes.
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页码:263 / 269
页数:7
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