LOCAL COMPARISON OF THE GENOMES OF MYCOBACTERIUM-TUBERCULOSIS AND MYCOBACTERIUM-LEPRAE USING THE POLYMERASE CHAIN-REACTION

被引:0
作者
PHILIPP, WJ [1 ]
COLE, ST [1 ]
机构
[1] INST PASTEUR, UNITE GENET MOLEC BACTERIENNE, F-75724 PARIS 15, FRANCE
关键词
MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; GENOME ORGANIZATION; GENOME MAP; MYCOBACTERIUM LEPRAE;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To facilitate comparison of the genome maps of Mycobacterium tuberculosis and M. leprae, sequence data from the M. leprae sequencing project were used to design primers suitable for the amplification of short segments from conserved genes by the polymerase chain reaction. In most cases, both organisms yielded products of identical size that were then used as probes for a comparative genomic walk. The hybridization data often, but not always, revealed a similar organization of various regions of both genomes. This approach should be useful for systematic comparisons of (myco)bacterial genomes.
引用
收藏
页码:263 / 269
页数:7
相关论文
共 50 条
[41]   Detection and resistance of Mycobacterium tuberculosis on rifampicin using nested polymerase chain reaction and sequencing method [J].
Rosilawati, Maria Lina .
UNIVERSA MEDICINA, 2007, 26 (01) :1-10
[42]   Detection of Mycobacterium leprae in ocular tissues by histopathology and real-time polymerase chain reaction [J].
Shamsi, Farrukh A. ;
Chaudhry, Imtiaz A. ;
Moraes, Milton O. ;
Martinez, Alejandra N. ;
Riley, Fenwick C. .
OPHTHALMIC RESEARCH, 2007, 39 (02) :63-68
[43]   Comparison of three different primer pairs for the detection of Mycobacterium tuberculosis by polymerase chain reaction in paraffin-embedded tissues [J].
Ozkara, HA ;
Kocagoz, T ;
Ozcelik, U ;
Akcoren, Z ;
Gocmen, A .
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 1998, 2 (06) :451-455
[44]   Comparison of in-house polymerase chain reaction method with the Roche Amplicor™ technique for detection of Mycobacterium tuberculosis in cytological specimens [J].
Mehrotra, R ;
Metz, P ;
Kohlhepp, S .
DIAGNOSTIC CYTOPATHOLOGY, 2002, 26 (04) :262-265
[45]   Comparison of in house polymerase chain reaction with conventional techniques for the detection of Mycobacterium tuberculosis DNA in granulomatous lymphadenopathy [J].
Singh, KK ;
Muralidhar, M ;
Kumar, A ;
Chattopadhyaya, TK ;
Kapila, K ;
Singh, MK ;
Sharma, SK ;
Jain, NK ;
Tyagi, JS .
JOURNAL OF CLINICAL PATHOLOGY, 2000, 53 (05) :355-361
[46]   Detection of Mycobacterium tuberculosis in preserved tuberculous lymph nodes by polymerase chain reaction [J].
Yang, B ;
Koga, H ;
Ohno, H ;
Ogawa, K ;
Hossain, MA ;
Fukuda, M ;
Hirakata, Y ;
Tomono, K ;
Tashiro, T ;
Kohno, S .
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 184 (02) :123-131
[47]   Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in solitary pulmonary nodules [J].
Shim, JJ ;
Cheong, HJ ;
Kang, EY ;
In, KH ;
Yoo, SH ;
Kang, KH .
CHEST, 1998, 113 (01) :20-24
[48]   Direct diagnosis of mycobacterium tuberculosis in blood samples of hiv infected patients by polymerase chain reaction [J].
Kamatchiammal S. ;
Saravanakumar D. ;
Kumarasamy N. ;
Solomon S. ;
Sritharan M. ;
Sritharan V. .
Indian Journal of Clinical Biochemistry, 2000, 15 (2) :76-82
[49]   POLYMERASE CHAIN-REACTION AMPLIFICATION OF SEGMENTS OF PESTIVIRUS GENOMES [J].
ROEHE, PM ;
WOODWARD, MJ .
ARCHIVES OF VIROLOGY, 1991, :231-238
[50]   DNA from Mycobacterium tuberculosis identified in Mediaeval human skeletal remains using polymerase chain reaction [J].
Taylor, GM ;
Crossey, M ;
Saldanha, J ;
Waldron, T .
JOURNAL OF ARCHAEOLOGICAL SCIENCE, 1996, 23 (05) :789-798