IN-VIVO DIFFERENTIATION OF LEUKOCYTES ROLLING IN MESENTERIC POSTCAPILLARY VENULES

被引:30
作者
TANGELDER, GJ
JANSSENS, CJJG
SLAAF, DW
EGBRINK, MGAO
RENEMAN, RS
机构
[1] UNIV LIMBURG, CARDIOVASC RES INST, DEPT PHYSIOL, 6200 MD MAASTRICHT, NETHERLANDS
[2] UNIV LIMBURG, CARDIOVASC RES INST, DEPT BIOPHYS, 6200 MD MAASTRICHT, NETHERLANDS
[3] AGR UNIV WAGENINGEN, DEPT HUMAN & ANIM PHYSIOL, 6709 PJ WAGENINGEN, NETHERLANDS
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1995年 / 268卷 / 02期
关键词
LEUKOCYTE MARGINATION; FLUORESCENCE; ACRIDINE YELLOW; MICROCIRCULATION; RABBITS; INTRAVITAL MICROSCOPY;
D O I
10.1152/ajpheart.1995.268.2.H909
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A method is presented to assess in vivo in transparent tissues the leukocyte subtypes that roll in microvessels. In nine rabbits anesthetized with ketamine-xylazine, leukocyte nuclei were stained in situ with acridine yellow (3 mg/kg iv for 5 min). Intravital fluorescence video microscopy in 24 mesenteric venules (17-29 mu m, median 21) indicated labeling of all rolling leukocytes. On the basis of the shape of their nucleus, 67-100% (median 89) could be classified unequivocally (13-366 cells analyzed, median 77) as polymorphonuclear (PMN, i.e., granulocytes) or monomorphonuclear (lymphocytes and monocytes). Of these classified cells, 94-100% were PMNs (median 100, including 1 stray value of 69%). This PMN percentage was independent of the level of leukocyte rolling (2-36/min, median 14), vessel diameter, flow velocity (0.5-2.5 mm/s), or duration of the experiment ( < 6 h). The dye had no significant influence on hemodynamic param eters, systemic leukocyte counts (1.5-7.8 x 10(9)/l), or in vitro differentiation pattern (27-38% granulocytes, 0-2% monocytes, 61-71% lymphocytes). In conclusion, our method demonstrated that the leukocytes that roll in postcapillary venules of the exteriorized rabbit mesentery are almost exclusively granulocytes.
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页码:H909 / H915
页数:7
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