CULTIVATION OF PLASMODIUM-FALCIPARUM PARASITES IN A SERUM-FREE MEDIUM

被引：42

作者：

OFULLA, AVO

OKOYE, VCN

KHAN, B

GITHURE, JI

ROBERTS, CR

JOHNSON, AJ

MARTIN, SK

机构：

[1] WALTER REED ARMY INST RES,DEPT HEMATOL,WASHINGTON,DC 20307

[2] KENYA GOVT MED RES CTR,CLIN RES CTR,NAIROBI,KENYA

[3] WALTER REED ARMY INST RES,USA MED RES UNIT KENYA,WASHINGTON,DC 20307

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1993年 / 49卷 / 03期

关键词：

D O I：

10.4269/ajtmh.1993.49.335

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The elimination of serum from Plasmodium falciparum culture media could decrease costs, enhance procurement, and improve the feasibility of large-scale production of parasite material. We provide a semi-defined, serum-free formulation, of commercially available constituents that supports P. falciparum parasite growth at rates comparable with those obtained with serum-supplemented media. The medium is composed of RPMI 1640 to which HEPES, extra glucose, bicarbonate, and hypoxanthine have been added. Bovine albumin and serum-derived, lipids-cholesterol-rich mixture are then used in place of serum.

引用

页码：335 / 340

页数：6

共 17 条

[1] SERUM-FREE CELL-CULTURE - A UNIFYING APPROACH [J].