IMMOBILIZATION OF DNA FOR SCANNING PROBE MICROSCOPY

被引：57

作者：

ALLISON, DP

BOTTOMLEY, LA

THUNDAT, T

BROWN, GM

WOYCHIK, RP

SCHRICK, JJ

JACOBSON, KB

WARMACK, RJ

机构：

[1] OAK RIDGE NATL LAB,DIV CHEM,OAK RIDGE,TN 37831

[2] OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37831

[3] GEORGIA INST TECHNOL,DEPT CHEM & BIOCHEM,ATLANTA,GA 30332

[4] UNIV TENNESSEE,GRAD SCH BIOMED SCI,KNOXVILLE,TN 37996

[5] UNIV TENNESSEE,DEPT PHYS,KNOXVILLE,TN 37996

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 21期

关键词：

D O I：

10.1073/pnas.89.21.10129

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented.

引用

页码：10129 / 10133

页数：5

共 33 条

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