CYTOTOXICITY WITH TARGET DNA BREAKDOWN BY RAT BASOPHILIC LEUKEMIA-CELLS EXPRESSING BOTH CYTOLYSIN AND GRANZYME-A

被引：233
作者：
SHIVER, JW
SU, LS
HENKART, PA
机构：
[1] NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892
[2] STANFORD UNIV,MED CTR,SCH MED,DEPT PATHOL,STANFORD,CA 94305
来源：
CELL | 1992年 / 71卷 / 02期
关键词：
D O I：
10.1016/0092-8674(92)90359-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The noncytotoxic rat mast cell tumor line RBL was transfected with genes for the cytotoxic lymphocyte granule proteins cytolysin (perforin) and granzyme A, giving transfectants with mRNA and protein expression levels comparable with cloned cytotoxic T lymphocytes. Both RBL-cytolysin and RBL-cytolysin-granzyme A transfectants showed extremely potent killing of red cell targets and lysed 20%-60% of EL4 lymphoma targets at an effector-to-target ratio of 30. RBL transfectants expressing only granzyme A were not cytotoxic. Significant EL4 DNA breakdown accompanying lysis was observed only with RBL that was transfected with both cytolysin and granzyme A. These results support the granule-exocytosis model for lymphocyte cytotoxicity and show that effector granzyme A plays a role in target cell DNA breakdown.
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收藏
页码：315 / 322
页数：8
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