CAPILLARY GEL-ELECTROPHORESIS FOR RAPID, HIGH-RESOLUTION DNA SEQUENCING

被引：247

作者：

SWERDLOW, H ^{[1
]}

GESTELAND, R ^{[1
]}

机构：

[1] UNIV UTAH, HOWARD HUGHES MED INST, DEPT BIOENGN, SALT LAKE CITY, UT 84132 USA

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 06期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/18.6.1415

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary gel electrophoresis has been demonstrated for the separation and detection of DNA sequencing samples. Enzymatic dideoxy nucleotide chain termination was employed, using fluorescently tagged oligonucleotide primers and laser based on-column detection (limit of detection is 6,000 molecules per peak). Capillary gel separations were shown to be three times faster, with better resolution (2.4 ×), and higher separation efficiency (5.4 ×) than a conventional automated slab gel DNA sequencing instrument. Agreement of measured values for velocity, resolution and separation efficiency with theory, predicts further improvements will result from increased electric field strengths (higher voltages and shorter capillaries). Advantages of capillary gel electrophoresis for automatic DNA sequencing instruments and for genomic sequencing are discussed. © 1990 Oxford University Press.

引用

页码：1415 / 1419

页数：5

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