CAP-DEPENDENT RNA SPLICING IN A HELA NUCLEAR EXTRACT

被引:146
作者
EDERY, I [1 ]
SONENBERG, N [1 ]
机构
[1] MCGILL UNIV, MCGILL CANC CTR, MONTREAL H3G 1Y6, QUEBEC, CANADA
关键词
D O I
10.1073/pnas.82.22.7590
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have studied the involvement of the 5'' cap structure in the splicing of precursor mRNAs in a HeLa nuclear extract. We show that precursor mRNAs are spliced efficiently only when they possess a cap structure and that preincubation of a HeLa nuclear extract rendered the splicing reaction highly sensitive to inhibition by cap analogues. This sensitization was dependent on exogenous Mg2+ but not exogenous ATP or GTP. These results demonstrate that splicing in a nuclear extract is highly dependent on the cap structure, as was demonstrated for the splicing process in a HeLa whole-cell extract [Konarska, M. M., Padget, R. A. and Sharp, P. A. (1984) Cell 38, 731-736], and thus support the contention that cap recognition is an important feature of eukaryotic mRNA biogenesis.
引用
收藏
页码:7590 / 7594
页数:5
相关论文
共 16 条
[1]   5'-TERMINAL CAP STRUCTURE IN EUKARYOTIC MESSENGER RIBONUCLEIC-ACIDS [J].
BANERJEE, AK .
MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :175-205
[2]   THE SPLICEOSOME - YEAST PRE-MESSENGER RNA ASSOCIATES WITH A 40S COMPLEX IN A SPLICING-DEPENDENT REACTION [J].
BRODY, E ;
ABELSON, J .
SCIENCE, 1985, 228 (4702) :963-967
[3]   ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].
DIGNAM, JD ;
LEBOVITZ, RM ;
ROEDER, RG .
NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489
[4]   ACCURATE AND EFFICIENT INVITRO SPLICING OF PURIFIED PRECURSOR RNAS SPECIFIED BY EARLY REGION-2 OF THE ADENOVIRUS-2 GENOME [J].
GOLDENBERG, CJ ;
HAUSER, SD .
NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1337-1348
[5]   A MULTICOMPONENT COMPLEX IS INVOLVED IN THE SPLICING OF MESSENGER-RNA PRECURSORS [J].
GRABOWSKI, PJ ;
SEILER, SR ;
SHARP, PA .
CELL, 1985, 42 (01) :345-353
[6]   SPLICING OF INVITRO SYNTHESIZED MESSENGER-RNA PRECURSORS IN HELA-CELL EXTRACTS [J].
HERNANDEZ, N ;
KELLER, W .
CELL, 1983, 35 (01) :89-99
[7]   RELATION BETWEEN INHIBITION OF PROTEIN-SYNTHESIS AND CONFORMATION OF 5'-PHOSPHORYLATED 7-METHYLGUANOSINE DERIVATIVES [J].
HICKEY, ED ;
WEBER, LA ;
BAGLIONI, C ;
KIM, CH ;
SARMA, RH .
JOURNAL OF MOLECULAR BIOLOGY, 1977, 109 (02) :173-183
[8]  
KONARSKA MM, 1984, CELL, V38, P731, DOI 10.1016/0092-8674(84)90268-X
[9]   CHARACTERIZATION OF THE BRANCH SITE IN LARIAT RNAS PRODUCED BY SPLICING OF MESSENGER-RNA PRECURSORS [J].
KONARSKA, MM ;
GRABOWSKI, PJ ;
PADGETT, RA ;
SHARP, PA .
NATURE, 1985, 313 (6003) :552-557
[10]   NORMAL AND MUTANT HUMAN BETA-GLOBIN PRE-MESSENGER RNAS ARE FAITHFULLY AND EFFICIENTLY SPLICED INVITRO [J].
KRAINER, AR ;
MANIATIS, T ;
RUSKIN, B ;
GREEN, MR .
CELL, 1984, 36 (04) :993-1005