PURIFICATION AND PRELIMINARY CHARACTERIZATION OF 3 C-TYPE CYTOCHROMES FROM PSEUDOMONAS-NAUTICA STRAIN-617

被引：8

作者：

SARAIVA, LM

BESSON, S

MOURA, I

FAUQUE, G

机构：

[1] UNIV AIX MARSEILLE 2,CTR OCEANOL MARSEILLE,CNRS,URA41,F-13288 MARSEILLE 09,FRANCE

[2] UNIV NOVA LISBOA,INST TECNOL QUIM & BIOL,P-2780 OEIRAS,PORTUGAL

[3] UNIV NOVA LISBOA,FAC CIENCIAS & TECNOL,DEPT QUIM,P-2825 MONTE DE CAPARICA,PORTUGAL

[4] UNIV NOVA LISBOA,FAC CIENCIAS & TECNOL,CTR QUIM FINA & BIOTECNOL,P-2825 MONTE DE CAPARICA,PORTUGAL

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three c-type cytochromes, namely cytochrome c(553), cytochrome c(553)(548) and Cytochrome c', were purified from the marine denitrifying bacterium Pseudomonos nautica strain 617. These three monohemic cytochromes present in small amounts were preliminarily characterized by physico-chemical and spectroscopic techniques. The visible and the H-1-NMR spectra show that cytochrome c(553) and cytochrome c(553)(548) have histidine-methionine as iron axial ligands. Cytochrome c(553) and cytochrome c(553)(548) have mid-paint redox potentials of +269 mV and +223 mV, at pH 7.6, and their molecular masses are 14 kDa and 17 kDa, respectively. Cytochrome c' has a molecular mass of 21 kDa and its visible spectrum is typical of a high spin heme. (C) 1995 Academic Press, Inc.

引用

页码：1088 / 1097

页数：10

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