Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells

被引:41
作者
Bandaruk, Yauhen [1 ]
Mukai, Rie [1 ]
Terao, Junji [1 ]
机构
[1] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Food Sci, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
来源
TOXICOLOGY REPORTS | 2014年 / 1卷
关键词
Monoamine oxidase-A; Quercetin; Luteolin; SH-SY5Y cells; Serotonin metabolism;
D O I
10.1016/j.toxrep.2014.08.016
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Monoamine oxidase-A (MAO-A) is the main enzyme in the metabolism of the neurotransmitter serotonin (5-hydroxytryptamine). Elevated activity of MAO-A in the brain may contribute to the pathogenesis of depressive disorders. Plant flavonoids, such as flavonol quercetin and flavone luteolin, have been suggested to be potential antidepressant compounds because they exert a suppressive effect on the MAO-A reaction. We evaluated the effects of these flavonoids on MAO-A activity and protein level using SH-SY5Y as model serotoninergic nerve cells. Quercetin and luteolin were incorporated into SH-SY5Y cells rapidly and converted to O-methylated derivatives. Luteolin accumulated in cells after 24-h incubation, whereas quercetin disappeared completely from cell fractions and culture medium. Addition of ascorbic acid prevented the disappearance of quercetin and allowed it to exert its cytotoxicity (similar to luteolin) at > 10 mu M. Luteolin and quercetin were incorporated into mitochondria fractions within 1-h incubation and attenuated MAO-A activity slightly but significantly. After 24-h incubation, luteolin attenuated MAO-A activity, but quercetin needed ascorbic acid for its attenuation. Neither luteolin nor quercetin significantly affected MAO-A protein level. These data suggest that luteolin and quercetin can be direct inhibitors of MAO-A in nerve cells by targeting mitochondria. (C) 2014 The Authors. Published by Elsevier Ireland Ltd.
引用
收藏
页码:639 / 649
页数:11
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