PLANT-REGENERATION THROUGH SOMATIC EMBRYOGENESIS IN QUERCUS-SUBER

被引:0
|
作者
BUENO, MA [1 ]
ASTORGA, R [1 ]
MANZANERA, JA [1 ]
机构
[1] UNIV POLITECN MADRID,ESCUELA TECN SUPER INGN MONTES,UNIDAD ANAT & FISIOL VEGETAL,E-28040 MADRID,SPAIN
关键词
CORK OAK; PLANT REGENERATION; QUERCUS-SUBER; SOMATIC EMBRYOGENESIS; TISSUE CULTURE;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cork oak (Quercus suber L.) zygotic embryos, endosperm and ovules were treated with different concentrations of 2,4-D for induction of somatic embryos. Plant material was collected during the embryo development season, from June to September. Immature embryos proved to be the most reactive initial explant. Callus and somatic embryos developed a few weeks after the beginning of the 2,4-D treatment. For embryo development experiments, different growth regulators and cold and desiccation treatments were tested. Cold storage of somatic embryos matured in vitro at 5-degrees-C was the best treatment for breaking dormancy.
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页码:30 / 34
页数:5
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