ESTABLISHMENT OF THE ANTIOXIDANT/ANTIRADICAL ACTIVITY OF THE INHIBITORS USING THE DPPH - RADICAL

This research paper presents the results of the investigation of antioxidant activities of various inhibitors, which are constituents of winery products: quercitin, rezveratrol, dihydroxyfumaric acid. Also, the antioxidant activity of tartaric and dihydroxyfumaric (DFH 4) acids derivatives has been determined: sodium dihydroxyfumarate, dimethylic ester of DFH 4 and dimethylic ester of tartaric acid. The enotannin extracts obtained from grape seeds have been evaluated: the non-oxidized enotannin extract Eneox and the oxidized one - Enoxil. For the determination of the antioxidant/antiradical activity the 2,2-diphenil-1-picrylhidrazil (DPPH) radical was used, which has the absorption maxima at 517 nm. The efficient concentration EC 50, the stoichiometric value of the antioxidant and the free radical, the antiradical power (1/EC 50) and the number of moles of DPPH-radical reduced by one mole of inhibitor have been calculated for all investigated inhibitors. It was found that catechin, quercitin and DFH 4 exhibit the highest inhibition rate.