CONTROLLING ELECTROOSMOTIC FLOW IN CAPILLARY ZONE ELECTROPHORESIS

被引：36

作者：

COHEN, N ^{[1
]}

GRUSHKA, E ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,DEPT INORGAN & ANALYT CHEM,IL-91904 JERUSALEM,ISRAEL

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 678卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)87086-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electroosmotic flow in capillary zone electrophoresis must be controlled for precise and reproducible performance. We found that the addition of any one of several classes of compounds such as amines, amino acids or organic acids to the running buffer stabilizes electroosmotic flow. With the additives in the buffer, the precision of migration times improved to better than 1%. The reproducibilities improved to better than 5%. The system is very stable at various conditions for small molecules and for proteins at very acidic or very basic conditions.

引用

页码：167 / 175

页数：9

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