IMMUNOELECTRON MICROSCOPIC DETECTION OF THE HEPATITIS-B VIRUS MAJOR SURFACE PROTEIN IN DILATED PERINUCLEAR MEMBRANES OF YEAST-CELLS

被引：16

作者：

BIEMANS, R ^{[1
]}

THINES, D ^{[1
]}

PETREPARENT, B ^{[1
]}

DEWILDE, M ^{[1
]}

RUTGERS, T ^{[1
]}

CABEZON, T ^{[1
]}

机构：

[1] CATHOLIC UNIV LOUVAIN,CELL BIOL LAB,B-1348 LOUVAIN,BELGIUM

来源：

DNA AND CELL BIOLOGY | 1992年 / 11卷 / 08期

关键词：

D O I：

10.1089/dna.1992.11.621

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The major surface protein of hepatitis B virus produced in Saccharomyces cerevisiae can be recovered from cell lysates in the form of 22-mm lipoprotein particles. Immunoelectron microscopy was applied to investigate site and time of particle assembly. Thin sections of yeast cells revealed that production of the S protein provoked a dilation of the endoplasmic reticulum. Dilated areas were specifically labeled with a polyclonal antibody raised against glutaraldehyde-treated yeast-derived HBsAg particles. In contrast to previous postulates of particle formation during cell lysis and extract preparation, these results suggest that particle formation in yeast occurs in the endoplasmic reticulum and that transport of particles along the secretion pathway is blocked.

引用

页码：621 / 626

页数：6

共 27 条

[1] TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID [J].

BEGGS, JD .

NATURE, 1978, 275 (5676) :104-109

[2] THE LARGE SURFACE PROTEIN OF HEPATITIS-B VIRUS IS RETAINED IN THE YEAST ENDOPLASMIC-RETICULUM AND PROVOKES ITS UNIQUE ENLARGEMENT [J].

BIEMANS, R ;

THINES, D ;

RUTGERS, T ;

DEWILDE, M ;

CABEZON, T .

DNA AND CELL BIOLOGY, 1991, 10 (03) :191-200

[3] HEPATITIS-B VACCINE PRODUCED IN YEAST [J].

BITTER, GA ;

EGAN, KM ;

BURNETTE, WN ;

SAMAL, B ;

FIESCHKO, JC ;

PETERSON, DL ;

DOWNING, MR ;

WYPYCH, J ;

LANGLEY, KE .

JOURNAL OF MEDICAL VIROLOGY, 1988, 25 (02) :123-140

[4] EXPRESSION OF HUMAN ALPHA-1-ANTITRYPSIN CDNA IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].

CABEZON, T ;

DEWILDE, M ;

HERION, P ;

LORIAU, R ;

BOLLEN, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (21) :6594-6598

[5] EXCRETION OF HEPATITIS-B SURFACE-ANTIGEN PARTICLES FROM MOUSE CELLS TRANSFORMED WITH CLONED VIRAL-DNA [J].

DUBOIS, MF ;

POURCEL, C ;

ROUSSET, S ;

CHANY, C ;

TIOLLAIS, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (08) :4549-4553

[6] CURING OF ENDOGENOUS 2 MICRON DNA IN YEAST BY RECOMBINANT VECTORS [J].

HARFORD, MN ;

PEETERS, M .

CURRENT GENETICS, 1987, 11 (04) :315-319

[7]

HARFORD N, 1983, DEV BIOL STAND, V54, P125

[8]

HARFORD N, 1987, POSTGRAD MED J, V63, P65

[9] EXPRESSION OF HEPATITIS-B VIRUS SURFACE-ANTIGEN IN YEAST [J].

HITZEMAN, RA ;

CHEN, CY ;

HAGIE, FE ;

PATZER, EJ ;

LIU, CC ;

ESTELL, DA ;

MILLER, JV ;

YAFFE, A ;

KLEID, DG ;

LEVINSON, AD ;

OPPERMANN, H .

NUCLEIC ACIDS RESEARCH, 1983, 11 (09) :2745-2763

[10] HIGH-LEVEL PRODUCTION AND ISOLATION OF HUMAN RECOMBINANT ALPHA-1-PROTEINASE INHIBITOR IN YEAST [J].

HOYLAERTS, M ;

WEYENS, A ;

BOLLEN, A ;

HARFORD, N ;

CABEZON, T .

FEBS LETTERS, 1986, 204 (01) :83-87

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