DETECTION OF INFECTIOUS BURSAL DISEASE VIRUSES USING INSITU HYBRIDIZATION AND NONRADIOACTIVE PROBES

被引：14

作者：

JACKWOOD, DJ ^{[1
]}

SWAYNE, DE ^{[1
]}

FISK, RJ ^{[1
]}

机构：

[1] OHIO STATE UNIV,DEPT VET PATHOBIOL,COLUMBUS,OH 43210

来源：

AVIAN DISEASES | 1992年 / 36卷 / 01期

关键词：

D O I：

10.2307/1591731

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The in situ hybridization assay was developed for the detection of infectious bursal disease virus (IBDV) infections in chickens. Bursal tissue samples were harvested 4 days following infection with the ST-C, MD, E, IN, or SAL IBDV strain. The cDNA clones STC-243, located on genome segment A, and STC-119, located on genome segment B, were used to prepare non-radioactive probes. Probes were labeled with digoxigenin and detected the homologous ST-C virus and also heterologous viruses in bursal tissue sections. No positive cells were observed in tissue sections from uninfected control chickens.

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页码：154 / 157

页数：4

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