Screening of poultry samples for Salmonella Typhimurium by PCR assay

被引：1
作者：
Anumolu, V. K. [1 ]
Lakkineni, V. R. [1 ]
机构：
[1] Sri Venkateswara Vet Univ, Coll Vet Sci, Dept Vet Publ Hlth, Hyderabad 500030, Andhra Pradesh, India
来源：
VETERINARY WORLD | 2012年 / 5卷 / 03期
关键词：
Poultry samples; fli C gene; PCR; Salmonella Typhimurium;
D O I：
10.5455/vetworld.2012.169-172
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Poultry samples viz., cloacal swabs, egg swabs, poultry faeces and feed were screened for Salmonella Typhimurium. A set of primers derived from fli C gene were employed to standardize PCR for detection of Salmonella Typhimurium from poultry samples, which gave specific amplification of a 620 bp fragment. Boiling and snap chilling method used for template preparation. Screening of 112 samples revealed that 12 samples positive for Salmonella Typhimurium by PCR assay.
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收藏
页码：169 / 172
页数：4
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