DETECTION OF HEPATITIS-C VIRUS-RNA IN SERUM OF PATIENTS WITH CHRONIC HEPATITIS-C TREATED WITH INTERFERON-ALPHA

被引：203

作者：

HAGIWARA, H ^{[1
]}

HAYASHI, N ^{[1
]}

MITA, E ^{[1
]}

UEDA, K ^{[1
]}

TAKEHARA, T ^{[1
]}

KASAHARA, A ^{[1
]}

FUSAMOTO, H ^{[1
]}

KAMADA, T ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT MED 1,FUKUSHIMA 1-1-50,FUKUSHIMA KU,OSAKA 553,JAPAN

来源：

HEPATOLOGY | 1992年 / 15卷 / 01期

关键词：

D O I：

10.1002/hep.1840150108

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We tested serial serum samples for hepatitis C virus RNA from patients undergoing treatment for chronic hepatitis C with interferon-alpha using an assay that combined reverse transcription and polymerase chain reaction. The subjects studied were 20 patients with chronic hepatitis who had serum antibody to hepatitis C virus (anti-C 100-3). Before therapy, hepatitis C virus RNA was detected in 18 (90%) and 20 (100%) patients using primer sets derived from the NS3 region or the 5'-noncoding region of hepatitis C virus, respectively. Hepatitis C virus RNA became undetectable in all patients whose ALT level fell into the normal range during therapy. However, hepatitis C virus RNA reappeared in all patients whose ALT levels rose again after therapy, usually before the relapse. In patients whose ALT levels did not become normal, hepatitis C virus RNA did not disappear during therapy. Thus therapy with interferon-alpha appears to be beneficial in chronic hepatitis C because of its suppressive effects on hepatitis C virus replication. Detection of hepatitis C virus RNA in serum is useful for evaluating the antiviral effect of interferon.

引用

页码：37 / 41

页数：5

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