TRANSCRIPTION FACTOR USF FROM DUCK ERYTHROCYTES TRANSACTIVATES EXPRESSION OF THE HISTONE H5 GENE INVITRO BY INTERACTING WITH AN INTRAGENIC SEQUENCE

被引：13
作者：
DURING, F
GERHOLD, H
SEIFART, KH
机构：
[1] Institut fur Molekularbiobgie und Tumorforschung, 1 0-3550 Marburg/Lahn, Karl-van-Fnsch-Straße
来源：
NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 05期
关键词：
D O I：
10.1093/nar/18.5.1225
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The duck histone H5 gene contains a 12 base pair (bp) sequence motif within the coding region, which shows homology in 10 out of 12 bp with the consensus sequence of the USF binding site in the Ad2ML- promoter. The functional equivalent of transcription factor USF, partially purified from whole cell extracts of duck erythrocytes (EUSF), was shown to interact with this Intragenic sequence. Electrophoretic mobility shift analyses revealed the selective formation of a complex between this protein fraction and the duck H5 gene. Footprint assays with DNase I delineated specific binding to the Intragenic sequence outlined above. Moreover this protein fraction, containing EUSF, transactivates the expression of the duck H5 gene in vitro and elimination of the USF-consensus sequence leads to a loss of stimulation but retains the basic transcription of the gene. These results suggest an as yet unknown functional role of EUSF in the expression of the H5 gene of the duck. © 1990 Oxford University Press.
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页码：1225 / 1231
页数：7
相关论文
共 33 条
[21]  
Maxam A M, 1980, Methods Enzymol, V65, P499
[22]   SPECIFIC INTERACTION BETWEEN A TRANSCRIPTION FACTOR AND THE UPSTREAM ELEMENT OF THE ADENOVIRUS-2 MAJOR LATE PROMOTER [J].
MIYAMOTO, NG ;
MONCOLLIN, V ;
EGLY, JM ;
CHAMBON, P .
EMBO JOURNAL, 1985, 4 (13A) :3563-3570
[23]   IDENTIFICATION OF A CYCLIC-AMP-RESPONSIVE ELEMENT WITHIN THE RAT SOMATOSTATIN GENE [J].
MONTMINY, MR ;
SEVARINO, KA ;
WAGNER, JA ;
MANDEL, G ;
GOODMAN, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (18) :6682-6686
[24]   THE 1ST INTRON OF THE HUMAN GROWTH-HORMONE GENE CONTAINS A BINDING-SITE FOR GLUCOCORTICOID RECEPTOR [J].
MOORE, DD ;
MARKS, AR ;
BUCKLEY, DI ;
KAPLER, G ;
PAYVAR, F ;
GOODMAN, HM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (03) :699-702
[25]   SP1, A CAAT-BINDING FACTOR, AND THE ADENOVIRUS MAJOR LATE PROMOTER TRANSCRIPTION FACTOR INTERACT WITH FUNCTIONAL REGIONS OF THE GAMMA-FIBRINOGEN PROMOTER [J].
MORGAN, JG ;
COURTOIS, G ;
FOUREL, G ;
CHODOSH, LA ;
CAMPBELL, L ;
EVANS, E ;
CRABTREE, GR .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (06) :2628-2637
[26]   FINE ANALYSIS OF THE ACTIVE H-5 GENE CHROMATIN OF CHICKEN ERYTHROID-CELLS AT DIFFERENT STAGES OF DIFFERENTIATION [J].
RENAUD, J ;
RUIZCARRILLO, A .
JOURNAL OF MOLECULAR BIOLOGY, 1986, 189 (01) :217-226
[27]   GENOMIC ORGANIZATION OF THE GENES-CODING FOR THE 6 MAIN HISTONES OF THE CHICKEN - COMPLETE SEQUENCE OF THE H5-GENE [J].
RUIZCARRILLO, A ;
AFFOLTER, M ;
RENAUD, J .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 170 (04) :843-859
[28]   INTERACTION OF A GENE-SPECIFIC TRANSCRIPTION FACTOR WITH THE ADENOVIRUS MAJOR LATE PROMOTER UPSTREAM OF THE TATA BOX REGION [J].
SAWADOGO, M ;
ROEDER, RG .
CELL, 1985, 43 (01) :165-175
[29]   POSITIVE AND NEGATIVE REGULATION OF THE GENE FOR TRANSCRIPTION FACTOR-IIIA IN XENOPUS-LAEVIS OOCYTES [J].
SCOTTO, KW ;
KAULEN, H ;
ROEDER, RG .
GENES & DEVELOPMENT, 1989, 3 (05) :651-662
[30]   PROTEIN FACTOR(S) BINDING INDEPENDENTLY TO 2 DIFFERENT REGIONS OF THE ADENOVIRUS-2 MAJOR LATE PROMOTER [J].
SHI, XP ;
LEE, R ;
WEINMANN, R .
NUCLEIC ACIDS RESEARCH, 1986, 14 (09) :3729-3744
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