DIRECT MICROTITRE PLATE ENZYME-IMMUNOASSAY OF TESTOSTERONE IN UNEXTRACTED SERUM

被引：14
作者：
DHAR, TK [1 ]
ALI, E [1 ]
机构：
[1] INDIAN INST CHEM BIOL,4 RAJA SC MULLICK RD,CALCUTTA 700032,W BENGAL,INDIA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 147卷 / 02期
关键词：
TESTOSTERONE; DIRECT ENZYME IMMUNOASSAY;
D O I：
10.1016/S0022-1759(12)80004-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for enzyme immunoassay of testosterone in serum has been developed which does not require extraction of the serum with organic solvents. The release of testosterone from the binding proteins is achieved by heating the serum at 70-degrees-C for 30 min in an alkaline buffer. The results correlated well (r = 0.96) with those of a radioimmunoassay using I-125-labelled testosterone and with enzyme immunoassay with prior extraction of samples (r = 0.98). The detection limit of the assay is 1 pg per well and the turn around time for 36 samples is 3.5 h. The procedure is simple and well suited for routine analysis.
引用
收藏
页码：167 / 172
页数：6
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共 13 条
  • [1] SOLID-PHASE RADIOIMMUNOASSAY OF ESTRADIOL-17BETA[J]. ABRAHAM, GE. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1969(06)
  • [2] AN ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) FOR PLASMA TESTOSTERONE[J]. ELDER, PA;LEWIS, JG. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1985(05)
  • [3] GALLATI H, 1985, J CLIN CHEM CLIN BIO, V23, P453
  • [4] HOWARD K, 1989, CLIN CHEM, V35, P2044
  • [5] ISMAIL AAA, 1976, HORMONE ASSAYS THEIR, P581
  • [6] SENSITIVE AND SPECIFIC ENZYMEIMMUNOASSAY FOR SERUM TESTOSTERONE[J]. JOSHI, UM;SHAH, HP;SUDHAMA, SP. STEROIDS, 1979(01)
  • [7] A SIMPLE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR TESTOSTERONE[J]. MARCUS, GJ;DURNFORD, R. STEROIDS, 1985(06)
  • [8] COMPARISON OF PROCEDURES FOR ATTACHING STEROIDAL GLUCOSIDURONIC ACIDS TO BOVINE SERUM-ALBUMIN[J]. MATTOX, VR;LITWILLER, RD;NELSON, AN. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1979(02)
  • [9] MEYER HHD, 1986, J STEROID BIOCH S, V25, P139
  • [10] A COMPETITIVE MICROTITRE PLATE ENZYME-IMMUNOASSAY FOR PLASMA TESTOSTERONE USING POLYCLONAL ANTI-TESTOSTERONE IMMUNOGLOBULINS[J]. RAJKOWSKI, KM;HANQUEZ, C;BOUZOUMOU, A;CITTANOVA, N. CLINICA CHIMICA ACTA, 1989(02)
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