ISOLATION AND SEQUENCE-ANALYSIS OF SEA-URCHIN (LYTECHINUS-PICTUS) HISTONE H4 MESSENGER-RNA

Histone messenger RNAs isolated from early blastula stage Lytechinus pictus sea urchin embryos were separated into discrete RNA bands on polyacrylamide gels. The most rapidly migrating of these molecules, the putative histone H4 mRNA, was digested with T1 ribonuclease to generate oligonucleotides for nucleotide sequence analysis. Many of these sequences are colinear with the highly conserved amino acid sequence of histone H4 protein as determined for both cows and peas. Histone H4 messenger RNA hybridizes in conditions of DNA excess to sea urchin DNA which is repeated approximately 470-fold. Despite this level of repetition the nucleotide sequence of the H4 messenger RNA reflects little evolutionary divergence within the H4 genes of L. pictus as judged by the stoichiometric yield of T1 oligonucleotides and the hybridization and thermal stability of histone H4 mRNA-DNA hybrids.