NEUTRALIZING DETERMINANTS DEFINED BY MONOCLONAL-ANTIBODIES ON POLYPEPTIDES SPECIFIED BY BOVINE HERPESVIRUS-1

Monoclonal antibodies were used to study neutralizing determinants on polypeptides of bovine herpesvirus 1. Two of 3 monoclonal antibodies which recognized nonoverlapping epitopes on a glycoprotein of 82,000 daltons were found to neutralize. A 2nd group of monoclonal antibodies that individually precipitated 5 viral glycopolypeptides ranging in size from 102,000-55,000 daltons also neutralized. Two monoclonal antibodies which were the most efficient in neutralization recognized a non-glycosylated protein of 115,000 daltons which was the major polypeptide on the virus. A 4th group of monoclonal antibodies precipitated a non-glycosylated polypeptide of 91,000 daltons and several smaller polypeptides, but these antibodies demonstrated only limited neutralizing activity.