IMMUNOHISTOCHEMICAL MICROQUANTITATION METHOD FOR TYPE-I COLLAGEN IN KIDNEY HISTOLOGICAL SECTION OF THE RATS

被引：9

作者：

MANABE, N ^{[1
]}

FURUYA, Y ^{[1
]}

NAGANO, N ^{[1
]}

MIYAMOTO, H ^{[1
]}

机构：

[1] KIRIN BREWERY CO LTD,PHARMACEUT RES LAB,TAKASAKI,GUNMA 37012,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1994年 / 56卷 / 01期

关键词：

immunohistochemical quantitation; kidney; type I collagen;

D O I：

10.1292/jvms.56.147

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An immunohistochemical micromethod for quantitation of type I collagen in frozen histological section of rat kidney was developed. A principle of this method is enzyme-linked immunosorbent assay (ELISA). The intensity of immunoreactivity of the section to an anti-type I collagen antibody was quantitated, and then the total protein content in the same section was measured colorimetrically. Type I collagen content was expressed as mg of type I collagen per g of total protein. In this micromethod, the minimum area and optimum thickness of the section were 5 mm2 and 10 mum, respectively. Type I collagen content in the rat kidney section was 3.02 +/- 0.12 mg/g. This method should be useful for diagnosis of renal failure.

引用

页码：147 / 150

页数：4

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